菱角壳分级萃取物对肿瘤细胞增殖及凋亡的影响

目的:制备菱角壳乙醇提取物的分级萃取物,并研究其对肿瘤细胞增殖及凋亡的作用。方法:采用有机溶剂萃取法将菱角壳乙醇提取物分为乙酸乙酯萃取物和正丁醇萃取物2个不同极性部分,并测定其黄酮与多酚含量,CCK-8法测定菱角壳分级萃取物对肿瘤细胞增殖的抑制作用,流式细胞术检测菱角壳萃取物诱导肿瘤细胞凋亡的作用。结果:乙酸乙酯萃取物中多酚和黄酮含量高于正丁醇萃取物。在两种萃取物浓度为25、50、100、200、400 μg/mL时均能抑制胃癌细胞(SGC-7901)、肝癌细胞(Hep G2)的增殖,最高抑制率均达60%以上,并存在明显的剂量与效应关系。两种萃取物均能促进肿瘤细胞凋亡,400 μg/mL的乙酸乙酯、正丁醇萃取物作用肝癌细胞24 h,凋亡率分别为45.57%和33.34%;作用胃癌细胞24 h,凋亡率分别为31.01%和21.65%。结论:菱角壳乙酸乙酯萃取物具有抑制肿瘤细胞增殖及促肿瘤细胞凋亡的生物活性。

Effects of Proliferation and Apoptosis by Water Chestnut Shell Extracts on Tumor Cells

Objective:To prepare fractionated extracts from extraction of water chestnut shell by ethanol and study the effects of fractionated extracts on proliferation and apoptosis of tumor cell.Methods:The ethanol extract of water chestnut shell was divided into ethyl acetate extract and n-butanol extract by organic solvent extraction.The contents of flavonoids and polyphenols were determined.The CCK-8 method and flow cytometry were used to detect the inhibition of proliferation and the the effect of water chestnut shell extract on the apoptosis of tumor cells.Results:The contents of polyphenols and flavonoids in ethyl acetate extract were higher than that of n-butanol extract.The concentrations of 25,50,100,200,400μg/mL extracts of two kinds all had inhibitory effects on the proliferation of Hep G2 cells and SGC-7901 cells.The highest inhibition rate was more than 60%,and the dose increased significantly.When the extract was used to treat tumor cells at 400μg/mL for 24 h,the cells showed significant apoptosis.The apoptotic rates of hepatoma cells treated with ethyl acetate and n-butanol extracts were 45.57%and 33.34%;the apoptotic rates of gastric cancer cells treated with ethyl acetate and n-butanol extracts were 31.01%and 21.65%.Conclusion:The ethyl acetate extract of the water chestnut shell had strong effects on inhibiting the proliferation of tumor cells and promoting the apoptosis of tumor cells.