大孔树脂纯化苜蓿叶蛋白肽的工艺优化

通过对比6种大孔树脂对苜蓿叶蛋白肽吸附效果,筛选最佳大孔吸附树脂,并研究其对苜蓿叶蛋白肽的吸附-解吸特性。试验结果表明,纯化苜蓿叶蛋白肽的最适大孔吸附树脂为DA201-C型,最佳工艺条件为:上样流速0.5mL/min,上样浓度10mg/mL,75%乙醇溶液为洗脱剂,洗脱流速0.5mL/min,洗脱体积200mL。在该条件下苜蓿叶蛋白肽含量较最初提高了46.95%,糖含量降低了81.88%,盐和其他杂质含量降低了70.97%。DA201-C型大孔吸附树脂对苜蓿叶蛋白肽有较好的纯化效果。

Optimization on purification technology of alfalfa leaf protein peptide bymacroporous adsoption resin

The best macroporous adsorption resin was selected by comparing the adsorption effects of six macroporous adsorption resins on Alfalfa Leaf Protein (ALPP), and the adsorption-desorption characteristics of ALPP were studied. The results showed that the best type of macroporous adsorption resin for purifying ALPP was DA201-C. The optimum conditions were as follows: flow rate of sampling was 0.5 mL/min, concentration of sampling was 10 mg/mL, 75% ethanol solution was an eluent, the elution flow rate was 0.5 mL/min and the elution volume was 200 mL. Under these conditions, content of ALPP inceased by 46.95%, content of sugar decreased by 81.88%, content of salt and other impurities decreased by 70.97%. DA201-C macroporous adsorption resin had better purification effect on ALPP.