海洋细菌GM-1-1菌株对白色念珠菌的抗菌作用机理初步研究

为了探究生防解淀粉芽孢杆菌(Bacillus amyloliquefaciens)GM-1-1菌株无菌发酵液对白色念珠菌(Candida albicans)的抗菌性质及其抗菌作用机理,采用牛津杯法检测其对白色念珠菌的抑菌效果,通过扫描电镜法、MTT法、分光光度法等测定发酵液对白色念珠菌孢子萌发、细胞形态、生物被膜形成、细胞通透性的影响,初步探讨其抗菌作用机理。结果表明,GM-1-1无菌发酵液对白色念珠菌具有明显的抑制作用,抑菌圈直径达39.5 mm,受抑制的白色念珠菌在不含发酵液的培养基上仍可继续生长;无菌发酵液对白色念珠菌的孢子萌发具有强烈的抑制作用;扫描电镜下发现处理后的白色念珠菌细胞表面凹陷,形态不规则,细胞壁残缺;无菌发酵液抑制了白色念珠菌生物被膜的形成,增加了细胞的通透性。本文初步揭示了GM-1-1无菌发酵液对白色念珠菌的抗菌作用机理,为该菌株的进一步研究和应用奠定基础。

Preliminary Study on the Antibacterial Mechanism of Marine Bacteria GM-1-1 against Candida albicans

In order to investigate the antibacterial properties of Bacillus amyloliquefaciens GM-1-1 sterile fermentation broth on Candida albicans and its antibacterial mechanism,the antibacterial effect against Candida albicans was detected by oxford cup method. Effects of fermentation broth on the spore germination,ultrastructure,biofilm determination ation and cell perrmeability of Candida albicans were determined by scanning electron microscopy,MTT method and spectrophotometry and the antibacterial mechanism was also discussed. The results showed that the GM-1-1 sterile fermentation broth had a significant inhibitory effect on Candida albicans,and the diameter of the inhibition zone was 39.5 mm. The inhibited Candida albicans continued to grow on the medium without the fermentation broth. The sterile fermentation broth had a strong inhibitory effect on the spore germination of Candida albicans. Scanning electron microscopy revealed a depression on the surface of the treated Candida albicans cells. The shape was irregular,and the cell wall was defective. The bacterial fermentation broth inhibited the formation of Candida albicans biofilm and increased the permeability of the cells. The paper preliminarily revealed the antibacterial mechanism of GM-1-1 sterile fermentation broth against Candida albicans,which laid a foundation for further research and application of the strain.