猕猴桃皮中6种多酚类化合物的HPLC检测法建立

建立使用高效液相色谱法同时测定猕猴桃皮中6种多酚类化合物含量的方法。检测方法为:色谱柱为InertSustain C18(4.6 mm×250 mm,5 μm),以甲醇-10 mmoL/L KH₂PO₄水溶液(pH2.0)为流动相进行梯度洗脱,柱温40 ℃,流速1 mL/min,进样量10 μL,检测波长为280 nm。结果表明:此方法可使6种目标组分在50 min内得到良好的分离,且方法重复性好(RSD≤1.40%)、稳定性较强(RSD≤2.86%)、精密度较高(RSD≤1.85%)、回收率在84.90~108.78%之间。该方法操作简单、快速,能为猕猴桃皮多酚的相关研究提供有效数据。

Development of Determination of 6 Polyphendic Compounds in Kiwifruit Peel by HPLC

The simultaneous determination method of six kinds of polyphenols content in kiwifruit peel was made by using the high performance liquid chromatography( HPLC).In terms of the specific approach,the InertSustain C18( 4.6 mm × 250 mm,5 μm) was applied to the chromatographic column. The gradient elution was carried out by using methanol-10 mmoL/L KH2PO4 aqueous solution( pH2.0) as mobile phase.The column temperature was 40 ℃,the flow rate was 1 m L/min,the sample volume was 10 μL,and the detection wavelength was detected at 280 nm. Results showed that this method could be used for separating the six target components well in 50 min.The approach had great reproducibility( RSD≤ 1.40%),strong stability( RSD≤ 2.86%) and high precision( RSD≤1.85%).Furthermore,the recovery rate was between 84.90% and 108.78%.Therefore,this was a simple and quick operation method which could effectively provide relevant data for the research of polyphones in kiwifruit peel.