山楂原花青素对人肝癌SMMC-7721细胞增殖和凋亡的影响

目的：探讨山楂原花青素提取物、表儿茶素和原花青素B2对人肝癌SMMC-7721细胞增殖及凋亡的影响。方法：体外培养SMMC-7721细胞，噻唑蓝法检测不同质量浓度山楂原花青素对细胞增殖的影响；4’,6-二脒基-2-苯基吲哚染色法观察细胞凋亡，Western blot法检测细胞凋亡相关蛋白的表达；检测每组细胞中过氧化氢酶（catalase，CAT）、超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、Na＋K＋-ATP酶活力和丙二醛（malondialdehyde，MDA）含量。结果：山楂原花青素提取物、表儿茶素和原花青素B2呈时间和质量浓度依赖性地显著抑制SMMC-7721细胞的增殖（P＜0.05），促进细胞凋亡；与对照组相比，SMMC-7721细胞中SOD、CAT、GSH-Px、Na＋K＋-ATP酶活力降低，MDA含量升高；细胞凋亡相关蛋白半胱天冬酶-8、半胱天冬酶-3、Bcl-2相关X蛋白表达量和多聚二磷酸腺苷核糖聚合酶片段化增加，Bcl-2表达量降低。结论：山楂原花青素可通过影响抗氧化酶活力和细胞凋亡相关蛋白来抑制SMMC-7721细胞增殖，促进细胞凋亡。

Effect of Hawthorn Proanthocyanidins on Proliferation and Apoptosis of Human Hepatocellular Carcinoma SMMC-7721 Cells

Objective: To investigate the effect of hawthorn proanthocyanidins extract, epicatechin and proanthocyanidin B2 on the proliferation and apoptosis of human hepatocellular carcinoma SMMC-7721 cells. Methods: SMMC-7721 cells were cultured in vitro. The effect of different concentrations of hawthorn proanthocyanidins on cell proliferation and apoptosis was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay and 4’,6-diamidino-2-phenylindole (DAPI) staining, respectively. The expression of apoptosis-related proteins was detected by Western blotting. The activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and Na+K+-ATPase and malondialdehyde (MDA) level in cells from each group were determined. Results: Hawthorn proanthocyanidins extract, epicatechin and proanthocyanidin B2 all significantly inhibited the proliferation of SMMC-7721 cells in a time-dependent and concentrationdependent manner (P < 0.05) and induced cell apoptosis. Compared with the control group, hawthorn proanthocyanidins extract, epicatechin and proanthocyanidin B2 decreased the activity of SOD, CAT, GSH-Px and Na+K+-ATPase and increased the level of MDA in SMMC-7721 cells. The expression of apoptosis-related proteins casepase-8, caspase-3, Bcl-2-associated X protein and the fragment of poly (ADP-ribose) polymerase were increased, while the expression of Bcl-2 was decreased. Conclusion: Hawthorn proanthocyanidins can inhibit the proliferation of SMMC-7721 cells and induce apoptosis through regulating antioxidant activity and apoptosis-related proteins.