2，4-二叔丁基苯酚对细叶小羽藓生理的影响

为探究木麻黄（Casuarina equisetifolia）林地化感物质中含量最为丰富的2,4-二叔丁基苯酚（2,4-di-tert-butylphenol,2,4-DTBP）对细叶小羽藓（Haplocladium microphyllum）生长发育的影响,用不同物质的量浓度（0,0.01,0.05,0.10,0.50,1.00 mmol·L^-1）的2,4-DTBP处理细叶小羽藓,分别进行叶绿素、脯氨酸和丙二醛（MDA）含量的测定.结果表明：用适宜物质的量浓度（0~0.10 mmol·L^-1）2,4-DTBP处理时,细叶小羽藓叶绿素含量增加,脯氨酸和丙二醛含量下降;而物质的量浓度大于0.50 mmol·L^-1时,叶绿素含量下降,脯氨酸和丙二醛含量增加.当2,4-DTBP浓度高于0.50 mmol·L^-1时,对细叶小羽藓产生明显的化感抑制效应,不利于细叶小羽藓生长.本研究以常见种细叶小羽藓为例,探讨了2,4-DTBP对苔藓植物的化感作用,为细叶小羽藓是否适应木麻黄林地生态环境提供参考.

Effects of 2, 4-di-tert-butyl phenol on physiology of Haplocladium microphyllum

In order to explore the effects of 2, 4-di-tert-butylphenol (2,4-DTBP), the most abundant allelochemicals in Casuarina equisetifolia forest, on the growth and development of Haplocladium microphyllum, we determined the content of chlorophyll, proline and malondialdehyde (MDA) at different mole concentrations (0, 0.01, 0.05, 0.10, 0.50 and 1.00 mmol∙L^-1) of 2,4-DTBP. The results showed that the appropriate concentration of 2,4-DTBP (0-0.10 mmol∙L^-1) increased the chlorophyll content of Haplocladium microphyllum and decreased the proline and MDA content, while the concentration of 2, 4-DTBP(>0.50 mmol∙L^-1) decreased the chlorophyll content and increased the proline and MDA content. When the concentration of 2,4-DTBP was higher than 0.50 mmol∙L^-1, it had an allelopathic inhibitory effect on Haplocladium microphyllum, which was not conducive to the growth of Haplocladium microphyllum. In this study, the allelopathic effect of 2,4-DTBP on bryophytes was discussed by taking Haplocladium microphyllum as an example, which provided a reference for exploring whether the Haplocladium microphyllum can adapt to the ecological environment of Casuarina equisetifolia forest land.