| A型激酶锚定蛋白在小鼠卵母细胞减数分裂阻滞中的作用 |
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| 引用本文： | 王述森,张震,罗阳,张阳.A型激酶锚定蛋白在小鼠卵母细胞减数分裂阻滞中的作用[J].中国医科大学学报,2012,41(7):588-590. |
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| 作者姓名： | 王述森 张震 罗阳 张阳 |
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| 作者单位： | 1. 中国医科大学基础医学院医学基因组学教研室,教育部医学细胞生物学重点实验室,沈阳110001
2. 中国医科大学附属第一医院超声科,沈阳,110001 |
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| 基金项目： | 国家自然科学基金青年基金资助项目(30800392);辽宁省教育厅高校科研计划(2008807) |
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| 摘 要： | 目的 探讨A型激酶锚定蛋白（AKAPs）在小鼠卵母细胞减数分裂阻滞中的作用。方法 收集生发泡期小鼠卵母细胞，显微注射Ht31或Ht31-P，观察卵母细胞减数分裂恢复情况。结果 在蛋白激酶A持续激活的情况下，Ht31注射组能促进小鼠卵母细胞减数分裂重新启动，解除蛋白激酶A引起的生发泡期阻滞，而Ht31-P对照组不能。结论 AKAPs介导的蛋白激酶A正确锚定对小鼠卵母细胞第一次减数分裂阻滞至关重要。
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| 关 键 词： | 卵母细胞 减数分裂阻滞 A型激酶锚定蛋白 |
| 收稿时间： | 2012-09-27; |
Dynamic Anchoring of PKA via AKAPs is Essential for Meiotic Resumption of Mouse Oocytes |
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| WANG Shu-sen
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ZHANG Zhen
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LUO Yang
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ZHANG Yang.Dynamic Anchoring of PKA via AKAPs is Essential for Meiotic Resumption of Mouse Oocytes[J].Journal of China Medical University,2012,41(7):588-590. |
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| Authors: | WANG Shu-sen ZHANG Zhen LUO Yang ZHANG Yang |
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| Affiliation: | 1(1.The Research Center for Medical Genomics,Key Laboratory of Medical Cell Biology,Ministry of Education,College of Basic Medical Science,China Medical University,Shenyang 110001,China;2.Department of Ultrasound,The First Hospital,China Medical University,Shenyang 110001,China) |
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| Abstract: | Objective To explore the role of A-kinase anchoring proteins(AKAPs) in meiotic arrest of mouse oocytes.Methods Ht31 or Ht31-P peptide was microinjected into GV-stage mouse oocytes that were incubated in the presence of dbcAMP.The GVBD rates were counted and the phosphorylation status of Cdc2-Tyr15 was determined by western blot.Results Ht31 stimulated oocyte maturation as indicated by GVBD and dephosphorylation of Cdc2-Tyr15,which overcame the dbcAMP-induced arrest.In the control group,the inactive Ht31-P had no effect on GVBD,and Cdc2-Tyr15 was phosphorylated.Conclusion PKA(protein kinase A) interaction with AKAPs is essential for the initial maintenance of meiotic arrest in mouse oocytes. |
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| Keywords: | oocytes meiotic arrest A-kinase anchoring proteins |
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