| 小鼠SFA DNA片段的克隆 |
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| 引用本文： | 桑一军,莫晓群,倪祖梅,施履吉.小鼠SFA DNA片段的克隆[J].分子细胞生物学报,1995(4). |
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| 作者姓名： | 桑一军 莫晓群 倪祖梅 施履吉 |
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| 作者单位： | 中国科学院上海细胞生物学研究所，中国科学院上海细胞生物学研究所，中国科学院上海细胞生物学研究所，中国科学院上海细胞生物学研究所 上海 200031，上海 200031，上海 200031，上海 200031 |
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| 摘 要： | 本工作将富集小鼠着丝粒DNA克隆于载体EMBL3,得到约2000个克隆,建立了着丝粒基因文库。从中随机挑取20个克隆,鉴定其克隆片段平均分子量大小约为14kb。
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| 关 键 词： | 富集着丝粒DNA 克隆 小鼠 |
THE MOLECULAR CLONING OF MOUSE CENTROMERIC DNA (SFA-DNA) |
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| Sang Yi-jun Mo Xiao-qun Ni Zu-mei Shi Lu-ji.THE MOLECULAR CLONING OF MOUSE CENTROMERIC DNA (SFA-DNA)[J].Journal of Molecular Cell Biology,1995(4). |
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| Authors: | Sang Yi-jun Mo Xiao-qun Ni Zu-mei Shi Lu-ji |
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| Abstract: | The centromeric DNA (SFA-DNA) isolated from enriched mouse centromeric preparation has been cloned. The vector adopted for the cloning was ELBL 3. About two thousand clones were obtained. The average size of the SFA-DNA inserts was
estimated by measuring the inserts obtained from a mixture of 20 radomly picked clones. It was found that the average size of the SFA-DNA inserts is approximately 14Kb. |
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| Keywords: | Centromeric DNA (SFA-DNA) Cloning Mouse |
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