用Midi MACS方法从白血病患者分选CD34+/CD123+细胞

本研究的目的是应用MidiMACS方法从急性髓系白血病（AML）患者骨髓细胞分选CD34^＋／CD123^＋细胞。首先。用淋巴细胞分离液从AML患者骨髓细胞分离单个核细胞（BMMNC），然后再通过MidiMACS方法相继分选出CD34^＋细胞．以及从CD34^＋细胞中分选出CD123^＋细胞并通过流式细胞术分析分选后细胞的富集度和回收率。结果显示：经过第一次分选后，CD34^＋细胞的富集度高达98.73％，平均为95．6％，其回收率最高可达到84．6％，平均为51％；第二次分选后，CD34^＋／CD123^＋细胞的富集度高达99．23％，平均约为83％；相对于分选前BMMNC而言。CD34^＋／CD123^＋细胞的回收率为34％，但相对于第一次分选后的CD34^＋细胞而言，其回收率为56％。结论：MidiMACS方法可用于AML患者骨髓CD34^＋／CD123^＋细胞的分选，分选后细胞的富集度和回收率与文献报道的通过FACS方法所获得的结果相类似。

CD34 +/CD123+ Cell Sorting from the Patients with Leukemia by Midi MACS Method

he aim of this study was to sort the CD34~+/CD123~+ cells from the bone marrow cells of patients with acute myeloid leukemia (AML) by Midi MACS method. Firstly, the bone marrow mononuclear cells (BMMNC) were isolated from the patients with AML with Ficoll Paque, CD34~+ cells were then isolated by Midi MACS method followed by the isolation of CD34~+/CD123~+ cells from the fraction of CD34~+ cells. The enrichment and recovery of CD34~+ and CD34~+/CD123~+ cells were assayed by FACS technique. The results showed that the enrichment of CD34~+ cells was up to 98.73%, its average enrichment was 95.6%, and the recovery of CD34~+ was 84.6%, its average recovery was 51% after the first round sorting, by the second round sorting, the enrichment of CD34~+/CD123~+ cells was up to 99.23%, its average enrichment was 83%. With regard to BMMNCs before sorting, the recovery of CD34~+/CD123~+ was 34%. But, on the CD34~+ cells obtained by the first round sorting,its recovery was 56%. In conclusion, these results confirmed that the method of Midi MACS sorting can be applied to sort CD34~+/CD123~+ cells from the bone marrow cells of AML patients, which give rise to the similar enrichment and recovery of the sorted cells with that of literature reported by the method of FACS.