| 杨树韧皮部次生代谢产物的提取与分析 |
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| 引用本文： | 孙守慧,李威,吕长利,高国平,祁金玉,许哲.杨树韧皮部次生代谢产物的提取与分析[J].植物研究,2009,29(1):120-123. |
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| 作者姓名： | 孙守慧 李威 吕长利 高国平 祁金玉 许哲 |
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| 作者单位： | 1.沈阳农业大学林学院,沈阳 110161;2.东北师范大学化学学院,长春 130024 |
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| 基金项目： | 国家自然科学基金“不同品系杨树次生代谢产物抗青杨天牛机理的研究”(No.30400342);;东北师范大学分析测试基金 |
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| 摘 要： | 通过不同化学方法提取并分析了不同品系杨树韧皮部10种次生代谢产物的含量及含量差别,研究了不同制样方法对单宁、酚类及酚酸产物含量提取结果的影响。研究结果表明：银中杨样品中苯酚及邻苯二酚的含量最高,欧洲黑杨最低;制样温度影响单宁的提取率,75℃下处理的样品最有利于单宁、酚类及酚酸类物质的提取;此外，分析实验结果还表明杨树韧皮部样品中没有生物碱存在或含量极低,超出检测范围。
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| 关 键 词： | 杨树 韧皮部 提取 次生代谢产物 |
Extraction and Analysis of Secondary Metabolites in Poplar Phloem |
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| SUN Shou-Hui,LI Wei,LÜ,Chang-Li,GAO Guo-Ping,Qi Jin-Yu,XU Zhe.Extraction and Analysis of Secondary Metabolites in Poplar Phloem[J].Bulletin of Botanical Research,2009,29(1):120-123. |
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| Authors: | SUN Shou-Hui LI Wei LÜ Chang-Li GAO Guo-Ping Qi Jin-Yu XU Zhe |
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| Affiliation: | 1.Shenyang Agricultural University;Shenyang 110161;2.Northeast Normal University;Changchun 130024 |
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| Abstract: | Ten secondary metabolites in phloem of different strains of poplars were extracted by different chemical methods and their contents were determined. The effect of different treatment process for samples on the extraction efficiency of tannin, phenols and phenolic acids was studied in detail. The results showed that the contents of phenol and pyrocatechol in Populus alba×P.berolinensis were maximum, while these values were minimum in P. nigra. The temperature of sample preparation had influence on the extraction efficiency of tannin. It was found that the sample treated at 75℃ had the optimal extraction result for tannin, phenol and phenolic acid constituents. In addition, the experimental results also indicated that there were no alkaloids in poplar phloem or their contents were too low to be detected within the detection limit of instrument. |
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| Keywords: | Poplar phloem extraction secondary metabolites |
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