Lactiplantibacillusplantarum ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome |
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Authors: | Young-Sil Lee Eun-Jung Park Gun-Seok Park Seung-Hyun Ko Juyi Park You-Kyung Lee Jong-Yeon Kim Daeyoung Lee Jihee Kang Hae-Jeung Lee |
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Affiliation: | 1.AtoGen Co., Ltd., Daejeon 34015, Korea; (G.-S.P.); (S.-H.K.); (J.P.); (Y.-K.L.); (D.L.); (J.K.);2.Department of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Korea; (E.-J.P.); (J.-Y.K.);3.Institute for Aging and Clinical Nutrition Research, Gachon University, Gyeonggi-do 13120, Korea |
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Abstract: | Obesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of Lactiplantibacillus plantarum ATG-K2 using 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. 3T3-L1 cells were incubated to determine the effect of lipid accumulation with lysate of L. plantarum ATG-K2. Mice were fed a normal fat diet or HFD with L. plantarum ATG-K2 and Orlistat for 8 weeks. L. plantarum ATG-K2 inhibited lipid accumulation in 3T3-L1 adipocytes, and reduced body weight gain, WAT weight, and adipocyte size in HFD-induced obese mice, concurrently with the downregulation of PPARγ, SREBP1c, and FAS and upregulation of PPARα, CTP1, UCP1, Prdm16, and ND5. Moreover, L. plantarum ATG-K2 decreased TG, T-CHO, leptin, and TNF-α levels in the serum, with corresponding gene expression levels in the intestine. L. plantarum ATG-K2 modulated the gut microbiome by increasing the abundance of the Lactobacillaceae family, which increased SCFA levels and branched SCFAs in the feces. L. plantarum ATG-K2 exhibited an anti-obesity effect and anti-hyperlipidemic effect in 3T3-L1 adipocytes and HFD-induced obese mice by alleviating the inflammatory response and regulating lipid metabolism, which may be influenced by modulation of the gut microbiome and its metabolites. Therefore, L. plantarum ATG-K2 can be a preventive and therapeutic agent for obesity. |
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Keywords: | obesity probiotics Lactiplantibacillus plantarum ATG-K2 gut microbiome short-chain fatty acids lipid metabolism |
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