| 红豆杉离体培养染色体数变异与细胞多核现象 |
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| 引用本文： | 余龙江,张长河,梅兴国,陈貌. 红豆杉离体培养染色体数变异与细胞多核现象[J]. 华中科技大学学报(自然科学版), 1998, 0(4) |
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| 作者姓名： | 余龙江 张长河 梅兴国 陈貌 |
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| 作者单位： | 华中理工大学生物工程系 |
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| 基金项目： | 国家“八五”科技攻关重点资助项目 |
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| 摘 要： | 分别对在固体培养基上静止培养和在摇瓶、１０Ｌ及１００Ｌ生物反应器内悬浮培养的红豆杉茎尖和根尖离体细胞进行染色体数目观察，发现其染色体数目主要类型和亲本株相同，为２ｎ＝２４，且存在较大比例的染色体数目变异的细胞．还发现，离体培养红豆杉细胞有丝分裂间期存在多核现象．认为染色体数目的变异可能是红豆杉离体培养细胞紫杉醇产量不稳定的主要原因．
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| 关 键 词： | 红豆杉；离体培养细胞；染色体数目变异；多核现象 |
Somaclonal Variation in the Number of Chromosome and the Number of Nuclei in Cultured Taxus chinensis Cells |
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| Yu Longjiang Assoc. Prof., Dept. of Bioengineering,HUST,Wuhan ,China Zhang Changhe Mei Xingguo Chen Mao. Somaclonal Variation in the Number of Chromosome and the Number of Nuclei in Cultured Taxus chinensis Cells[J]. JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE, 1998, 0(4) |
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| Authors: | Yu Longjiang Assoc. Prof. Dept. of Bioengineering HUST Wuhan China Zhang Changhe Mei Xingguo Chen Mao |
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| Affiliation: | Yu Longjiang Assoc. Prof., Dept. of Bioengineering,HUST,Wuhan 430074,China Zhang Changhe Mei Xingguo Chen Mao |
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| Abstract: | By counting the number of chromosome of explant and in vitro Taxus chinensis cells cultured in agar solidified medium, flasks, 10L and 100L bioreactors, the number of chromosome was found to be 2 n =24. Aneuploid cells with 6 and 18 chromosomes, triploid cells and tetraploid cells were also found. Cells with binuclei or tetranuclei were common, and cells having heptanuclei were also seen occasionally. Moreover, the sizes of nuclei in a cell were equal. It is suggested that instability of the number of chromosome may be the main cause of the oscillation in taxol production during long term subculture. |
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| Keywords: | Taxus chinensis in vitro cells variation in the number of chromosomes polynuclei . |
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