| 冻融和冻融+苏打盐碱复合胁迫下苜蓿幼苗的生理响应 |
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| 引用本文： | 李红,李波,方志坚,杨曌,张超,冯成龙,金姗姗,王思琦,石婉莹. 冻融和冻融+苏打盐碱复合胁迫下苜蓿幼苗的生理响应[J]. 草地学报, 2021, 29(7): 1469-1476. DOI: 10.11733/j.issn.1007-0435.2021.07.012 |
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| 作者姓名： | 李红 李波 方志坚 杨曌 张超 冯成龙 金姗姗 王思琦 石婉莹 |
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| 作者单位： | 1. 黑龙江省农业科学院畜牧兽医分院, 黑龙江 齐齐哈尔 161005;2. 齐齐哈尔大学生命科学与农林学院, 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江 齐齐哈尔 161006;3. 齐齐哈尔市园艺研究所, 黑龙江 齐齐哈尔, 161006 |
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| 基金项目： | 黑龙江省省属高等学校基本科研业务费科研项目（135209267，YSTSXK201886）；国家牧草产业技术体系齐齐哈尔综合试验站（CARS-34）；2021年大学生创新创业训练计划项目（202110232012，202110232156）资助 |
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| 摘 要： | 为了研究苜蓿(Medicago Sativa L.)幼苗在冻融和冻融+苏打盐碱胁迫下生理反应,以'龙牧807'苜蓿幼苗为试材,对其进行冻融循环[10℃(T-F10)→5℃(T-F5)→0℃(T-F0)→ —3℃ →0℃(F-T0)→5℃(F-T5)→10℃(F-T10)]和冻融循环+苏打盐碱(150 mmol·L-1)...
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| 关 键 词： | 苜蓿 冻融+苏打盐碱胁迫 生理响应 隶属函数 灰色关联度 |
| 收稿时间： | 2021-01-22 |
Physiological Response of Alfalfa Seedlings to Freeze-thaw or Freeze-thaw Versus Soda Saline-alkali Stress |
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| LI Hong,LI Bo,FANG Zhi-jian,YANG Zhao,ZHANG Chao,FENG Cheng-long,JIN Shan-shan,WANG Si-qi,SHI Wan-ying. Physiological Response of Alfalfa Seedlings to Freeze-thaw or Freeze-thaw Versus Soda Saline-alkali Stress[J]. Acta Agrestia Sinica, 2021, 29(7): 1469-1476. DOI: 10.11733/j.issn.1007-0435.2021.07.012 |
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| Authors: | LI Hong LI Bo FANG Zhi-jian YANG Zhao ZHANG Chao FENG Cheng-long JIN Shan-shan WANG Si-qi SHI Wan-ying |
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| Affiliation: | 1. Institute of Animal Husbandry and Veterinary of Heilongjiang academy of Agricultural sciences, Qiqihar, Heilongjiang Province 161005, China;2. College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang Province 161006, China;3. Institute of Horticulture in Qiqihar, Qiqihar, Heilongjiang Province, 161006, China |
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| Abstract: | In order to study the physiological response of alfalfa seedlings under freeze-thaw and freeze-thaw+soda saline alkali stress，'Longmu 807’ alfalfa seedlings were used as experimental materials to explore the physiological and biochemistry response of alfalfa to artificial simulated freeze-thaw cycles [10℃(T-F10)→5℃(T-F5)→0℃(T-F0)→—3℃→0℃(F-T0)→5℃(F-T5)→10℃(F-T10)] and freeze-thaw cycles + soda saline-alkali(150 mmol·L-1)stress. The effects of freeze-thaw cycle and soda saline alkali on physiological and biochemical characteristics of alfalfa leaves were discussed. The results showed that:the contents of soluble sugar,proline,soluble protein and the activities of peroxidase and superoxide dismutase in alfalfa leaves increased first and then decreased during the whole cycle of thawing (T-F) and freezing-thawing (F-T) and freezing-thawing cycle + soda saline alkali stress,and reached the maximum at —3℃. The activities of catalase,ascorbate peroxidase and relative electrical conductivity increased gradually. The membership function method was used to analyze the 9 physiological and biochemical indexes,we found that the membership values were —3℃ > F-T0 > F-T10 > F-T5 > T-F0 ＞ T-F5 > T-F10 > CK under freeze-thaw cycles stress,and were —3℃ + saline alkali > F-T0 + saline alkali > F-T10 + saline alkali > F-T5 + saline alkali > T-F0 + saline alkal > CK + saline alkali > T-F5 + saline alkali > T-F10 + saline alkali > CK under freeze-thaw + soda saline-alkali stress.The comprehensive correlation degree of freeze-thaw and freeze-thaw + soda saline-alkali stress with each index from high to low was proline > superoxide dismutase > catalase > soluble sugar > ascorbate peroxidase > soluble protein > malondialdehyde > peroxidase > relative conductivity. |
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| Keywords: | Alfalfa Freezing-thawing and soda saline-alkali stress Physiological response Membership function analysis Grey relational degree |
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