油菜蜂花粉提取物对羟基自由基介导2-脱氧核糖损伤的抑制作用

本文以水、80%乙醇、70%甲醇为溶剂提取油菜蜂花粉中的多酚类物质,测定了提取物中总酚含量、还原力、Fe2+络合能力及对DPPH自由基的清除能力。以Fenton为模型,研究了油菜蜂花粉提取物对羟基自由基介导2-脱氧核糖降解的保护机理。结果表明:3种提取物的抗氧化能力与总酚含量呈正相关,乙醇提取物和甲醇提取物的抗氧化能力较水提取物强;油菜蜂花粉提取物主要是作为自由基清除剂,不是依靠络合能力来发挥清除羟基自由基的作用。

Inhibitory Effect of Rape Bee Pollen Extracts on Hydroxyl Radical-mediated 2-deoxy-D-ribose Oxidative Damage

Polyphenols were extracted with water, 80%ethanol, 70%methanol from rape bee pollen (RBP). The total phenoliccontent of extracts was determined and the antioxidant properties of extracts were assayed using different methods including reducing power, iron (II) chelation and DPPH radical and Fenton chemistry system. The mechanism of scavenging effects of RBP extracts on hydroxyl radical-mediated 2-deoxyribose degradation was also studied. The results revealed that statistically significant relationship between total phenolic content of the three different extracts and antioxidant activity was found. Ethanolic and methanolic extracts from RBP showed higher antioxidant activities than water extracts. It was also found that RPB extracts had higher scavenging activity than iron chelation ability for hydroxyl radicals.