Antagonistic,synergistic, and additive antibacterial interaction between ciprofloxacin and amoxicillin against Staphylococcus aureus |
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| Authors: | Eduardo Gómez-Sánchez Lorenzo Franco-de la Torre Adriana Hernández-Gómez Ángel Josabad Alonso-Castro Nicolás Serafín-Higuera Flavio Terán-Rosales Ronell Eduardo Bologna-Molina Mario Alberto Isiordia-Espinoza |
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| Affiliation: | 1. Departamento de Ciencias Fisiológicas, División de Disciplinas Básicas para la Salud, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Tepatitlán de Morelos, Jalisco, Mexico;2. Instituto de Investigación en Ciencias Médicas, Cuerpo Académico Terapéutica y Biología Molecular (UDG-CA-973), Departamento de Clínicas, División de Ciencias Biomédicas, Centro Universitario de los Altos, Universidad de Guadalajara, Tepatitlán de Morelos, Jalisco, Mexico;3. Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, Mexico;4. Facultad de Odontología, Universidad Autónoma de Baja California, Mexicali, Baja California, Mexico;5. Sección de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, Mexico;6. Patología Molecular, Facultad de Odontología, Universidad de la República (UDELAR), Montevideo, Uruguay |
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| Abstract: | The aim of this in vitro study was to evaluate the interaction between ciprofloxacin and amoxicillin against beta-lactamase-producing Staphylococcus aureus. Concentration-dependent curves for each individual drug were carried out to obtain the mean inhibitory concentration in the agar well diffusion assay. Then, different ratios of the ciprofloxacin–amoxicillin combination (0.5:0.5, 0.8:0.2, 0.2:0.8, 0.9:0.1, 0.1:0.9, 0.95:0.05, and 0.05:0.95) were assessed. Data were analyzed using the isobolographic analysis and interaction index. The isobolographic evaluation shows that the 0.9:0.1 and 0.95:0.05 ratios of the ciprofloxacin–amoxicillin combination produced a synergistic antimicrobial interaction, the 0.8:0.2, 0.2:0.8, 0.1:0.9, and 0.05:0.95 proportions showed an additive antibacterial effect, and the 0.5:0.5 proportion induced antagonistic antimicrobial effects. The interaction index showed similar outcomes to the isobolographic analysis. In conclusion, the data of this study mainly show antimicrobial additive results of the ciprofloxacin–amoxicillin combination against beta-lactamase-producing S. aureus. |
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| Keywords: | amoxicillin antimicrobial interaction ciprofloxacin Staphylococcus aureus |
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