电针对脑缺血再灌注大鼠神经黏蛋白表达和脑含水量的影响

目的观察电针对易卒中型肾血管性高血压大鼠脑（RHRSP）缺血后再灌注不同时间神经黏蛋白表达、脑组织含水量的干预作用。方法将180只SD大鼠先用环形银夹狭窄双侧肾动脉,制成RHRSP,再用线栓法制成一侧大脑中动脉闭塞（MCAO）脑缺血再灌注模型。随机分为空白组、假手术组、电针组和对照组,分别观察缺血2h后再灌注1d、7d、14d、30d大鼠神经黏蛋白、脑组织含水量的变化。结果脑缺血再灌注1d,对照组脑缺血区周围出现神经黏蛋白阳性表达细胞,7d明显增多,14d表达到高峰,30d下降。电针组神经黏蛋白阳性表达细胞在7d、14d、30d时较对照组明显减少（P〈0.05或P〈0.01）,脑缺血1d、7d,电针组大鼠脑组织含水量明显小于对照组（P〈0.05或P〈0.01）。结论电针减轻脑缺血再灌注损伤后脑水肿,促进缺血损伤区神经功能修复,可能与其下调神经抑制因子神经黏蛋白的表达机制密切相关。

Effect of Electric Acupuncture on the Expression of Neurocan and Cerebral Water Content after Cerebral Ischemia and Reperfusion in Rats

Objective To study the effect of electric acupuncture on the expression of neurocan and cerebral water content of cerebral ischemia and reperfusion at the different time in stroke - prone - renovaseular - hypertensive rats（RHRSP）. Methods Total of 180 RHRSP models were tablished with ring - shape silver clipby on renal arteries. The models with focal cerebral ischemia and reper- fusion（I/R） of MCAO by intraluminal middle cerebral artery occlusion were categorized randomly into sham- operation group, electric acupuncture group and control group. The changes of neurocan and cerebral water content were detected at 1 day, 7 day, 14 day and 30 day. Results The positive cells of neurocan were found in ischemic area after 1 day ischemia and reperfusion in control group. It was increased significantly after 7 day reperfusion and it was increased to the maximum after 14 day reperfusion. But it was decreased after 30 day rcperfusion. Compared with control group, the expression of neurocan in electric acupuncture group was decreased at 7 day, 14 day and 30 day. The cerebral water content（80.72± 1.37） and（79.43± 1.25） in SPRHR group were markedly lower than that in control group（83.63-± 1.33） and（81.65±1.65） after 1 day and 7 day ischemia（P〈0.05 or P〈0.01）. Conclusion The electric acupuncture could promote brain edema and neural function recovery in SPRHR group,and it was related with the down regulation to the expression of neurocan.