大孔树脂HPD400分离茶皂素的研究

研究了大孔树脂HPD400分离茶皂素的方法。以硅胶柱色谱方法制备得到了茶皂素对照样品,在此基础上建立了比色法测定茶皂素含量的方法。以静态吸附与洗脱方法初步筛选HPD系列大孔树脂,进一步以动态吸附与乙醇梯度洗脱的方法研究了HPD400树脂分离纯化茶皂素的条件。实验结果表明:HPD400大孔树脂的动态饱和吸附容量为109.3 mg/g树脂,30%乙醇洗脱物茶皂素的含量为93.1%,50%乙醇洗脱物茶皂素的含量为87.1%;乙醇的总洗脱率达到80.3%。茶皂素主要由30%的乙醇洗脱,所得样品中茶皂素含量高,HPD400大孔树脂适合茶皂素的分离纯化。

Study on separation of tea saponin by macroporous resin HPD400

The method for separation of tea saponin was investigated. A standard sample of tea saponin was obtained by silica gel column chromatography and the method of colorimetric analysis of tea saponin was then established. HPD series of macroporous resin were screened by static adsorption and elution of ethanol,and the content was high. The separation and purification of tea saponin with HPD400 macroporous resin by dynamic adsorption and ethanol gradient elution. The results indicated that the dynamic saturated adsorption capacity of HPD400 was 109.3 mg/g and the content of tea saponin of sample eluted by 30% ethanol and 50% ethanol were 93.1% and 87.1%, respectively. The total elution rate with ethanol was 80.3%. Tea saponin was eluted mainly by 30% ethanol,and the content was high. HPD400 macroporous resin is suitable for separation and purification of tea saponin.