| 复合酶协同超声波法提取香椿老叶总黄酮工艺研究 |
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| 引用本文： | 刘玉梅,张家俊,吴浪.复合酶协同超声波法提取香椿老叶总黄酮工艺研究[J].现代食品科技,2019,35(11):223-230. |
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| 作者姓名： | 刘玉梅 张家俊 吴浪 |
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| 作者单位： | 铜仁职业技术学院,中兽药国家民委重点开放实验室,贵州铜仁554300 |
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| 基金项目： | 贵州省科学技术基金项目(黔科合J字[2014]2160)；铜仁市科技局科技计划项目(铜市科研(2013)10-13) |
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| 摘 要： | 本文研究了香椿老叶中总黄酮的提取工艺,提取液中总黄酮的得率以芦丁为对照品进行校正。先利用复合酶水解香椿老叶中的纤维素和果胶,探究出最佳酶解条件为复合酶2(果胶酶:纤维素酶=2:1),酶用量为2.00%(W/V),酶解时间40 min,酶解温度45℃,上清液中总黄酮得率为2.49%。再进行超声辅助提取,采用通过单因素实验和Box-Behnken实验对乙醇体积比、超声功率、超声时间、超声温度4个条件进行优化,各项方差分析表明影响香椿老叶中总黄酮得率的顺序依次为:超声功率乙醇体积分数提取温度提取时间,再根据回归方程预测最佳提取条件。在采用乙醇体积分数60%、超声功率227 W、提取温度53.00℃、提取时间51 min条件下,香椿老叶总黄酮得率为3.85%,与预测值仅相差0.03%,表明工艺参数准确可靠。
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| 关 键 词： | 香椿 总黄酮 酶解 超声波 响应面 |
| 收稿时间： | 2019/7/16 0:00:00 |
Extraction of Total Flavonoids from Toona sinensis Old Leaves by Complex Enzymolysis Assisted with Ultrasound |
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| LIU Yu-mei,ZHANG Jia-jun and WU Lang.Extraction of Total Flavonoids from Toona sinensis Old Leaves by Complex Enzymolysis Assisted with Ultrasound[J].Modern Food Science & Technology,2019,35(11):223-230. |
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| Authors: | LIU Yu-mei ZHANG Jia-jun and WU Lang |
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| Affiliation: | (Key Open Laboratory of Chinese Veterinary Medicine of State Ethnic Affairs Commission, Tongren Polyechnic College, Tongren 554300, China),(Key Open Laboratory of Chinese Veterinary Medicine of State Ethnic Affairs Commission, Tongren Polyechnic College, Tongren 554300, China) and (Key Open Laboratory of Chinese Veterinary Medicine of State Ethnic Affairs Commission, Tongren Polyechnic College, Tongren 554300, China) |
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| Abstract: | |
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| Keywords: | Toona sinensis flavonoid enzymatic hydrolysis ultrasonic response surface method |
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