| CRISPR/Cas9基因编辑技术在病原微生物中的应用 |
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| 引用本文： | 安鼎杰,康元环,陈龙,张海月,张冬星,田佳鑫,贾俊鹏,孙武文,单晓枫,钱爱东. CRISPR/Cas9基因编辑技术在病原微生物中的应用[J]. 中国人兽共患病杂志, 2017, 0(3): 280-286. DOI: 10.3969/j.issn.1002-2694.2017.03.017 |
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| 作者姓名： | 安鼎杰 康元环 陈龙 张海月 张冬星 田佳鑫 贾俊鹏 孙武文 单晓枫 钱爱东 |
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| 作者单位： | 吉林农业大学动物科学技术学院,长春,130118 |
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| 基金项目： | 国家自然科学基金项目(31201927)，吉林省重点科技攻关项目(20150204065NY)，the National Natural Science Foundation of China(31201927)，the Key Scientific and Technological Project of Jilin Provincial Science & Technology Department(20150204065NY) |
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| 摘 要： | 规律成簇间隔短回文重复序列(Clustered regularly interspaced short palindromic repeats,CRISPR)广泛存在于古细菌及细菌中是细菌在长期进化过程中形成的一种获得性免疫系统。近年来以该系统为基础,经过人工改造形成的一种新型基因编辑技术—CRISPR/Cas9在基因工程领域的应用越发广泛;该技术与前两代编辑技术相比,具有结构简单、成本低廉、实用价值较高等优点。自2012年在基因研究领域成功应用后,已成为当前关注度较高的基因编辑工具;该技术在多种真核生物的基因修饰中已得到成功应用,但在病原微生物上却报道较少。本文将从CRISPR/Cas9系统的结构、作用机制及其在病原微生物基因功能研究中的应用等几个方面进行综述为其深入研究奠定基础。
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| 关 键 词： | 基因编辑 CRISPR/Cas9 病原生物 脱靶效应 |
Advance and application of CRISPR/ Cas9 mediated genome editing technique on pathogenic microorganism |
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| AN Ding-jie,KANG Yuan-huan,CHEN Long,ZHANG Hai-yue,ZHANG Dong-xing,TIAN Jia-xin,JIA Jun-peng,SUN Wu-wen,SHAN Xiao-feng,QIAN Ai-dong. Advance and application of CRISPR/ Cas9 mediated genome editing technique on pathogenic microorganism[J]. Chinese Journal of Zoonoses, 2017, 0(3): 280-286. DOI: 10.3969/j.issn.1002-2694.2017.03.017 |
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| Authors: | AN Ding-jie KANG Yuan-huan CHEN Long ZHANG Hai-yue ZHANG Dong-xing TIAN Jia-xin JIA Jun-peng SUN Wu-wen SHAN Xiao-feng QIAN Ai-dong |
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| Abstract: | Clustered regularly interspaced short palindromic repeats (CRISPR) is an acquired immune system existing in archaea and bacteria with the long-term process of evolutionary.CRISPR/Cas9 gene editing system is a new type of gene editing technology developed based on the system.CRISPR/Cas9 is a more efficient method for gene targeting than the previous methods.It has been successfully applied for gene-modified of eukaryotes since 2012,but the reports about pathogenic microorgaisms are rarely.Here,the research progress in the structure,mechanism of CRISPR/Cas9 system and its applications on pathogenic microorgaisms is reviewed. |
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| Keywords: | geneome editing CRISPR/Cas9 pathogenic microorgaisms off-target effect |
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