| ADAM15在舌癌耐药细胞系Tca8113／ADM表达的实验研究 |
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| 引用本文： | 黄敏,邝晓聪,姚金光,关艳.ADAM15在舌癌耐药细胞系Tca8113／ADM表达的实验研究[J].广东牙病防治,2013(11):579-582. |
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| 作者姓名： | 黄敏 邝晓聪 姚金光 关艳 |
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| 作者单位： | [1]右江民族医学院口腔系,广西百色533000 [2]广西医科大学病理教研室,广西百色533000 |
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| 基金项目： | 国家自然科学基金（30860326）;广西壮族自治区教育厅科研项目[桂教科研（2005）47号] |
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| 摘 要： | 目的研究舌癌阿霉素（adriamycin；ADM）耐药细胞系Tca8113／ADM及其亲本细胞系Tca81t3肿瘤转移相关蛋白去整合素一金属蛋白酶15（adamalysin15，ADAM15）的表达水平，从肿瘤耐药的角度对舌癌的耐药与ADAM15的关系进行初步探讨。方法用蛋白免疫印迹技术检测Tca8113／ADM及Tca8113亲本细胞系的AD-AM15蛋白表达水平。结果ADAM15在Tca8113亲本细胞系和Tea8113／ADM细胞系的表达分别为0．244±0．231和1．217±0．494，两者差异具有统计学意义（P=0．001）。结论肿瘤转移相关蛋白ADAMt5在ToaS113／ADM细胞系的表达高于其在亲本细胞的表达，提示舌癌的阿霉素耐药细胞系的转移一洼可能比其亲本细胞更强。
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| 关 键 词： | 舌癌 ADAM15 Tca8113IADM 耐药 蛋白免疫印迹法 |
Expression of ADAM15 in Tca8113/ADM line |
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| HUANG Min,KUANG Xiao-cong,YAO Jin-guang,GUAN Yan.Expression of ADAM15 in Tca8113/ADM line[J].Journal of Dental Prevention and Treatment,2013(11):579-582. |
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| Authors: | HUANG Min KUANG Xiao-cong YAO Jin-guang GUAN Yan |
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| Affiliation: | ( Department of Stomatology, Youjiang Medical University for Nationalities, Baise 533000, China) |
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| Abstract: | Objective To detect the expression level of Adamalysinl5 （ ADAM15 ） of Tca8113 adriamycin drug-resistant tongue cancer cell line （Tca8113/ADM） of tongue cancer and Tca8113 parental cell line. Methods The expression level of ADAM15 of Tca8113/ADM cell line of tongue cancer and TcaSll3 parental cell line was detected by Western blotting, and then the relationship between the expression of ADAM15 in Tca8113 drug-resistant of tongue cancer and oral cancer metastasis was a.n_alysed. Results Compared with the expression of ADAM15 in Tca5113 parental cells, the expression of ADAM15 of Tca5113/ADM ceils was higher, and the difference was statistically significant （ P 〈 0.01 ）. Conclusion The results indicate that the metastasis potential ability of Tea8113/ADM cell line may be stronger than the Tca8113 parental cell line. |
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| Keywords: | Tongue cancer AdamalysinlS Tea8113/ADM Drug-resistant Western blotting |
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