蛇毒抗高凝状态酶对小鼠骨髓造血系统的影响

目的:研究蛇毒抗高凝状态酶(AHCSE)对实验性小鼠骨髓造血系统的影响和作用机制。方法:将小鼠随机分为四组,分别用AHCSE,阿霉素,AHCSE+阿霉素,生理盐水经尾静脉注射,10d后观察并测定血清白细胞介素-3(IL-3)含量,骨髓粒细胞比例及粒红比,脾系数。结果:蛇毒抗高凝状态酶能刺激骨髓造血细胞显著增生,血清IL-3含量,骨髓粒细胞比例及粒红比,脾系数AHCSE较其他三组明显增加,有统计学意义(P<0.05)。结论:AHCSE能显著刺激骨髓增生可能与激活细胞免疫机制使细胞因子IL-3增加有关。

Effects of antihypercoagulability state enzyme of snake venom on bone marrow hematopoietic system in mice

Objective:To investigate the proliferative effects and mechanism of the antihypercoagulability state enzyme on bone marrow hematopoietic systems in experimental mice.Methods:40 mice were randomly allocated to four groups by intravenous injection of AHCSE,ADM,AHCSE with ADM or normal saline respectively.The contents of interleukin-3(Multi-CSF),percentage of bone marrow granular leukocytes and G/E and spleen body ratio were determined in ten days.Results:AHCSE could stimulate myeloid elements to hyperplasy and increase the granular leukocytes percentage and G/E and spleen body ratio in comparison with the control groups,which had significant statistical difference(P<0.05).Conclusion:AHCSE can obviously stimulate bone marrow hemopoietic systems to hyperplasy,which may be correlated with the interleukin-3 multiplication by active cell immunologic mechanism.