| 无标记免疫分析——表面等离子体共振检测方法研究 |
| |
| 引用本文： | 李子颖,刘一兵,韩世泉,许文革,崔大付,李辉,王于杰.无标记免疫分析——表面等离子体共振检测方法研究[J].原子能科学技术,2009,43(2):108-112. |
| |
| 作者姓名： | 李子颖 刘一兵 韩世泉 许文革 崔大付 李辉 王于杰 |
| |
| 作者单位： | 1.中国原子能科学研究院 ;同位素研究所，北京 ;102413； ;2.中国科学院 ;电子学研究所 ;传感技术国家重点实验室，北京 ;100080 ; |
| |
| 摘 要： | 利用表面等离子体共振生物传感器对磺胺嘧啶及磺胺二甲嘧啶进行快速无标记检测研究，优化反应条件。抗原固定缓冲液pH为4.2~4.8；抗体浓度为1∶100～1∶200倍稀释；再生液为0.1mol/LH3PO4溶液，再生时间为1.5min。研究建立了标准曲线，并对芯片的稳定性进行了考察。
|
| 关 键 词： | 表面等离子谐振 磺胺嘧啶 磺胺二甲嘧啶 |
| 修稿时间： | 2008-07-25 |
No-Labeled Immunoassay by Using Surface Plasmon Resonance Biosensor |
| |
| LI Zi-ying,LIU Yi-bing,HAN Shi-quan,XU Wen-ge,CUI Da-fu,LI Hui,WANG Yu-jie.No-Labeled Immunoassay by Using Surface Plasmon Resonance Biosensor[J].Atomic Energy Science and Technology,2009,43(2):108-112. |
| |
| Authors: | LI Zi-ying LIU Yi-bing HAN Shi-quan XU Wen-ge CUI Da-fu LI Hui WANG Yu-jie |
| |
| Affiliation: | 1.China Institute of Atomic Energy, P. O. Box 275-39, Beijing 102413, China;2.State Key Laboratory of Transducer Technology, Institute of Electronics, ;Chinese Academy of Sciences, Beijing 100080, China ; |
| |
| Abstract: | Surface plasmon resonance biosensor was used to develop fast no-labeled immunoassays for sulfamethazine and sulfadiazine. The immobilization conditions of the antigen were studied and the working concentration of antibody and the condition of regeneration were optimized. Standard curves were set up and the stability of the chip was studied. |
| |
| Keywords: | surface plasmon resonance sulfamethazine sulfadiazine |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《原子能科学技术》浏览原始摘要信息 |
|
点击此处可从《原子能科学技术》下载全文 |
|
