Microcalorimetric investigation of the growth of the engineering <Emphasis Type="Italic">BACILLUS</Emphasis><Emphasis Type="Italic">THURINGIENSIS</Emphasis> with different plasmid numbers and various promoters期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Microcalorimetric investigation of the growth of the engineering BACILLUSTHURINGIENSIS with different plasmid numbers and various promoters

Authors:	L.?Xiaoyan,L.?Yi,S.?Ming,L.?Peng,Z.?Juncheng,L.?Lin,Q.?Songsheng,Y.?Ziniu author-information" > author-information__contact u-icon-before" > mailto:yz@public.wh.hb.cn" title=" yz@public.wh.hb.cn" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	(1) State Key Laboratory of Agricultural Microbiology of China, Huazhong Agricultural University Wuhan, 430070, P. R. China;(2) Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University Wuhan, 430072, P. R. China

Abstract:	A microcalorimetric technique based on the bacterial heat output was applied to evaluate the influence of plasmid copy number and various promoters on the green fluorescent protein expression in Bacillus thuringiensis. The thermogenic curves of the aerobic metabolism of B. thuringiensis strains were determined by using an LKB-2277 bioactivity monitor at 28°C. The analysis of the thermogenic curves indicated for the first time that the more plasmid copy number per cell the more protein synthetization. Promoter BtI-BtII had a stronger impact on the gene expression than promoter 3A investigated by the method of microcalorimetry.

Keywords:	microcalorimetry engineering strain green fluorescent protein
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