新疆药桑叶、枝多糖中单糖的组成分析与测定

目的:分析新疆药桑叶、枝多糖中单糖的组成并对其进行测定。方法:采用水提醇沉法提取新疆药桑叶、枝中的多糖,用2mol·L-1H2SO4将多糖水解成单糖,以1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化;采用反相高效液相色谱法测定各单糖。结果:桑叶、桑枝多糖均是由甘露糖、鼠李糖、葡糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖等单糖组成。7种单糖在25min内可很好地分离。结论:本方法操作简便、灵敏度高、结果准确,可用于新疆药桑的质量控制。

Analysis and Determination of the Composition of Monosaccharide in Polysaccharide from Leaves and Twigs of Morus nigra from Xinjiang

OBJECTIVE： To analyze and determine the compositions of monosaccharide in polysaccharide from leaves and twigs of Morus nigra from Xinjiang .METHODS： The polysaccharides in leaves and twigs of M.nigra were extracted by water and precipitated by ethanol then hydrolyzed into monosaccharide by using 2 mol· L^-1 H2SO4.The monosaccharide was derivatized with 1 - phenyl - 3 - methyl - 5 - pyrazolone（PMP） and determined by RP - HPLC. RESULTS： The polysaccharide in leaves and twigs of M. nigra were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose etc. All the 7 monosaccharides were well- separated within 25 min. CONCLUSION： The method is simple and sensitive with accurate results, and it can be used for the quality control of leaves and twigs of M. nigra from Xinjiang.