首页 | 官方网站   微博 | 高级检索  
     

新型介孔羟基磷灰石/壳聚糖-万古霉素药物释放系统复合材料的制备及体外抗菌和成骨能力
引用本文:张静哲,李冬冬,刘桂峰,吴琼,王金成.新型介孔羟基磷灰石/壳聚糖-万古霉素药物释放系统复合材料的制备及体外抗菌和成骨能力[J].高等学校化学学报,2012,33(2):219-224.
作者姓名:张静哲  李冬冬  刘桂峰  吴琼  王金成
作者单位:1. 吉林大学中日联谊医院,长春,130033
2. 吉林大学无机合成与制备化学国家重点实验室,长春,130012
基金项目:吉林大学无机合成与制备国家重点实验室开放课题基金
摘    要:采用冷冻干燥法合成了介孔羟基磷灰石(HA)/壳聚糖(CS)-万古霉素(VCM)药物释放系统复合材料, 利用SEM, XRD和FTIR等方法对材料进行了表征. 结果证实CS与HA混合复合材料具有良好的孔径和孔隙率, 万古霉素吸附于复合材料的表面和内部. 细胞毒性实验噻唑蓝(MTT)比色法]结果表明, 材料可以促进成骨细胞增殖且具有良好的细胞相容性. 体外抑菌实验结果证实此材料可长时间抑制耐甲氧西林金葡菌(MRSA)的生长, 具有良好的抑菌和杀菌能力. 细胞黏附实验结果表明, 成骨细胞附着于材料表面增殖并通过孔道延伸. 实时聚合酶链式反应(RT-PCR)实验结果表明, 在成骨相关标志产物胶原蛋白-1(COL-1)及骨形态发生蛋白-2(BMP-2)基因上均有较高的表达, 表明材料在体外可以促进成骨细胞生长, 具有良好的成骨能力.

关 键 词:羟基磷灰石  壳聚糖  药物释放  体外抗菌能力
收稿时间:2011-09-29

In vitro Evaluation of Antibacterial and Osteogenic Properties and Synthesis of Mesoporous Hydroxyapatite/Chitosan Composite Loaded with Vancomycin as a Drug Delivery
ZHANG Jing-Zhe , LI Dong-Dong , LIU Gui-Feng , WU Qiong , WANG Jin-Cheng.In vitro Evaluation of Antibacterial and Osteogenic Properties and Synthesis of Mesoporous Hydroxyapatite/Chitosan Composite Loaded with Vancomycin as a Drug Delivery[J].Chemical Research In Chinese Universities,2012,33(2):219-224.
Authors:ZHANG Jing-Zhe  LI Dong-Dong  LIU Gui-Feng  WU Qiong  WANG Jin-Cheng
Affiliation:1* (1.China-Japan Union Hospital,Jilin University,Changchun 130033,China; 2.State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,Jilin University,Changchun 130012,China)
Abstract:Mesoporous hydroxyapatite/chitosan(HA/CS) composite loaded with vancomycin(VCM) was synthesized using a freeze-drying method,and characterized by SEM,XRD and FTIR.The results showed that mesoporous HA/CS presents higher surface area and porosity.The concentration of vancomycin released from mesoporous HA/CS,the efficacy of the mesoporous HA/CS against methicillin-resistant Staphylococcus aureus(MRSA),the effect of mesoporous HA/CS on early adhesion,proliferation and differentiation of osteoblast cells in vitro were measured.Antibacterial assays indicated that mesoporous HA/CS loaded with vancomycin had the effect on growth of organisms and keep the diameter of inhibition zone until it disappeared for 13 d.MTT cytotoxicity test and cell adhesion test show that the materials have good biocompatibility and make cell viability and proliferation.In vitro testing using osteoblast-like cells show that the surfaces of the cells are able to adhere,proliferate,and migrate through the pores.These cells maintained similar expression levels of osteoblastic-associated markers,namely collagen type Ⅰ(COL-1),alkaline phosphatase(ALP),bone morphogenetic protein-2(BMP-2).It can be concluded that the mesoporous HA/CS loaded with vancomycin possesses good antibacterial capability and osteogenic properties in vitro and represents a promising candidate for bone regeneration as a drug delivery.
Keywords:Hydroxyapatite  Chitosan  Drug delivery  In vitro antibacterial property
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《高等学校化学学报》浏览原始摘要信息
点击此处可从《高等学校化学学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号