超高效液相色谱法测定卷烟烟丝中7种水溶性糖含量

以ACQUITY UPLC BEH Amide柱为分析柱，乙腈－0．2％三乙胺一水作为流动相，建立了超高效液相色谱－蒸发光散射检测器测定卷烟烟丝中鼠李糖、木糖、果糖、甘露糖、葡萄糖、蔗糖、麦芽糖7种水溶性糖的分析方法。7种水溶性糖回归方程的线性相关系数均大于0．999，检出限为0．56～1．11μg／mL。7种水溶糖的回收率在90．43％106．41％之间，相对标准偏差为1．66％－4．35％仰：6）。该方法灵敏度、准确度高，稳定性好，可用于大批量卷烟烟丝中水溶性糖含量的测定。

Determination of Carbohydrates in Cigarette by Ultra Performance Liquid Chromatography

A new method for the determination of seven carbohydrates such as rhamnose, xylose, fructose, mannose, glucose, sucrose, maltose in cigarette by ultra performance liquid chromatography with evaporative light scattering detector was established. The carbohydrates were separated on acquity UPLC BEH Amide column by using acetonitrile-0.2% TEA-water as the mobile phase. The linear regression coefficients of seven carbohydrates were more than 0.999, detection limits were 0.56-1.11μg/mL. Recoveries of the method for the seven carbohydrates were 90.4%-106.4% with relative standard deviations of 0.78%-5.66 %（n=6）. This method is sensitive and accurate, it can he applied to the determination of carbohydrates in cigarette.