3个不同地理群体真鲷遗传变异的RAPD分析

随机扩增多态DNA(random amplified polymorphic DNA,RAPD)是建立在PCR技术基础上的检测DNA序列多态性和建立分子遗传标记的技术,Williams等[1]首次运用随机引物扩增寻找多态DNA片段作为分子遗传标记.Welsh等[2]也发现以寡核苷酸作为引物对基因组DNA进行扩增,产物的图谱表现出高度的变异性.因其具有操作简单、能够快速高效地提供许多个体或基因型许多位点的DNA序列多态性数据等优点,在生物的遗传多样性、群体遗传学、分类学及农牧业的遗传育种等研究中得到了广泛的应用.

Genetic diversity of three geographical populations of Pagrosomus major revealed by RAPD analysis

Random amplif ied polymorphic DNA ( RAPD) technique was applied to assess the genetic variations among inter- and intra-population of three wild stocks( 24 individuals) in Red Sea Bream from Jiaozhou Gulf (Qingdao ) , Taiwan Strait ( Xiamen) and Beibu Gulf ( Hainan) . Under predetermined optimal reaction conditions, amplifications w ith 31 random primers selected from 60 gave 216 reproducible and stable fragments ranging f rom 200 to 2500bp. There were 176 polymorphic fragment s. Based on the RAPD data, the genetic distances of intrapopulat ion for Jiaozhou Gulf , Taiwan Strait and Beibu Gulf wild stocks were 0. 1350, 0. 1056, and 0. 1151, and similarit ies were 0. 8650, 0. 8944, and 0. 8849. The genet ic distances of interpopulation were 0. 1645, 0. 1413, and 0. 1073. The maximum occurred between Jiaozhou Gulf and Beibu Gulf , the minimum did between Taiwan Strait and Beibu Gulf . This showed there were high genet ic diversity among inter- and intrapopulation. With the methods of UPGMA and NJ in MEGA2. 1 on the basis of genetic distances, the results showed the populations of Taiwan Strait and Beibu Gulf assembled one branch first , then did Jiaozhou Gulf. Both results were accordant .