Detection and Quantitation of Bryostatin 1 and 2 in Bugula Neritina by Combined High-Peformance Liquid Chromatography and 3H-Phorbol Dibutyrate Displacement

Abstract

An HPLC method has been developed to detect and quantitate bryostatins 1 and 2 from crude extracts of B. neritina. This, coupled with a ³H-phorbol dibutyrate binding assay and photodiode array-acquired uv spectra makes possible the evaluation of crude extracts in a rapid, sensitive and specific manner.