| 姜黄素在Caco-2细胞模型中的吸收机制研究 |
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| 引用本文： | 闫文丽,黄兆胜,曾晓会,赵自明,黄雪君,房财富.姜黄素在Caco-2细胞模型中的吸收机制研究[J].现代食品与药品杂志,2011(11). |
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| 作者姓名： | 闫文丽 黄兆胜 曾晓会 赵自明 黄雪君 房财富 |
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| 作者单位： | 广州中医药大学;广东省中医研究所; |
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| 基金项目： | 广东省中医药局课题(编号:2009368) |
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| 摘 要： | 目的测定姜黄素透过Caco-2单细胞层的浓度,研究其吸收特征。方法用Caco-2细胞单层模型来考察时间、浓度、不同药物酮康唑、维拉帕米、胡椒碱对姜黄素吸收的影响。采用液相色谱-质谱联用(LC-MS)法,测定姜黄素浓度并计算其表观渗透系数(Papp)。结果不同浓度姜黄素在90 min之前Papp大小顺序为:180>240>300μg/ml;与对照组比较,胡椒碱,低浓度酮康唑,维拉帕米对姜黄素的吸收均呈不同程度的促进作用(P<0.05)。结论姜黄素的吸收具有明显的高浓度饱和现象,且不呈线性变化,初步判定其吸收为主动吸收机制;促进姜黄素吸收的原因可能与酮康唑抑制Ⅰ相代谢酶CYP450 3A4和1A2酶的活性;胡椒碱抑制姜黄素Ⅱ相代谢酶—葡萄糖醛酸苷酶;维拉帕米抑制药物外排性转运蛋白—P-糖蛋白的活性有关。
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| 关 键 词： | 姜黄素 Caco-2细胞模型 吸收 代谢酶 P-糖蛋白 |
Absorption Mechanism of Curcumin across Caco-2 Cell Model |
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| YAN Wen-li,HUANG Zhao-sheng,ZENG Xiao-hui,ZHAO Zi-ming,HUANG Xue-jun,FANG Cai-fu.Absorption Mechanism of Curcumin across Caco-2 Cell Model[J].JOurnal of Modern Food and Pharmaceuticals,2011(11). |
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| Authors: | YAN Wen-li HUANG Zhao-sheng ZENG Xiao-hui ZHAO Zi-ming HUANG Xue-jun FANG Cai-fu |
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| Affiliation: | YAN Wen-li1,HUANG Zhao-sheng1,ZENG Xiao-hui2,ZHAO Zi-ming2,HUANG Xue-jun2,FANG Cai-fu1(1.Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong 510405,China,2.Guangdong Provincial Institute of Traditional Chinese Medicine,Guangdong 510095,China) |
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| Abstract: | Objective To determine the concentration of curcumin and study the absorption mechanism of curcumin in Caco-2 cell monolayer model.Methods Caco-2 cell monolayer model was applied to investigate the effects of conveying times,drug concentration,and other different drug,such as ketoconazole,verapamil and piperine on the absorption of curcumin.LC/MS was used to determine the concentration of curcumin and the apparent permeability coefficient(Papp) was calculated.Results The Papps of curcumin at different conce... |
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| Keywords: | curcumin Caco-2 cell model absorption metabolic enzymes P-glycoprotein |
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