乌司他丁对大鼠实验性溃疡性结肠炎的作用

目的 探讨乌司他丁对大鼠实验性溃疡性结肠炎(UC)的作用及机制.方法 120只SD大鼠随机分为4组,每组各30只.使用三硝基苯磺酸钠( TNBS)建立UC模型,Ⅰ组不使用乌司他丁,为造膜对照组；Ⅱ、Ⅲ、Ⅳ组分别于造模0、6、12 h使用乌司他丁,剂量为2万单位/kg,为研究组.检测分析各组肠静脉内毒素、肿瘤坏死因子α(TNF-α)和UC模型症状积分(实验开始24、72、168 h各观察10只大鼠).结果 ①在实验各时段,使用乌司他丁组UC大鼠病变积分均低于Ⅰ组(均P＜0.05),且24、72 h时Ⅱ、Ⅲ、Ⅳ组差异均有统计学意义24 h:(1.8±0.4),(2.7±0.3),(3.0±0.1)；72 h:(3.7±2.3),(4.2±1.2),(5.0±0.9),均P＜0.05],但168 h时Ⅱ、Ⅲ、Ⅳ组未见明显差异.②使用乌司他丁组肠静脉内毒素含量在实验各时期内均低于Ⅰ组,24h时Ⅱ组与Ⅲ组、Ⅳ组之间差异均有统计学意义(97.7±11.7) ng/L比(117.1±9.7) ng/L,( 124.3 ±4.9) ng/L,均P＜0.05],72、168 h时Ⅱ、Ⅲ、Ⅳ组肠静脉血内毒素含量差异无统计学意义.③24h时Ⅱ组TNF-α低于Ⅰ组及Ⅲ、Ⅳ组(6.7±2.6) pg/100 μg比(13.5±1.7),(11.8±2.8),(12.4±2.7)pg/100 μg,均P＜0.05)],但72、168 h使用乌司他丁各组低于Ⅰ组.结论 乌司他丁对TNBS导致的大鼠UC有明显防护作用,其机制可能通过抑制内毒素水平而降低免疫异常水平.

The effect of ulinastatin on ulcerative colitis of rats

Objective To investigate the effect of ulinastatin on ulcerative colitis of rats. Methods One hundred and twenty sprague dawley （ S-D ） rats were divided into four groups randomly. Ulcerative colitis （ UC ） model was induced by three nitrobenzene sulfonate （TNBS）. The group I was control group and was free of ulinastatin ; group Ⅱ, Ⅲ, Ⅳ were given ulinastatin 2×10^5 u/（ kg·12 h） ] at 0, 6, 12 h after TNBS. Endotoxin, tumor necrosis factor （TNF）-α of Intestinal vein and UC symptomatic index were detected and analyzed. Results ①UC symptomatic index in given ulinastatin groups was lower than that in Ⅰ group at any stage （P 〈 0. 05 ） and showed statistical differences among group Ⅱ and Ⅲ, Ⅳ （ P 〈 0.05 ） at 24 h and 72 h. ②Endotoxin in ulinastatin groups was lower compared to that in control group （P 〈 0. 05 ）. ③ There was a lower TNF-α level in group Ⅱ compared to other groups （P 〈0. 05） ; at 72 h, 168 h, TNF-α in ulinastatin groups was lower than that in group Ⅰ （P 〈 0.05 ）. Conclusion Ulinastatin shows good effects on rats UC caused by TNBS. It may depress abnormal immune reaction through inhibiting endotoxin.