| HPLC法同时测定乳安凝胶膏剂中4种化学成分的含量 |
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| 引用本文： | 常星洁,刘志辉,严冬,傅静娟.HPLC法同时测定乳安凝胶膏剂中4种化学成分的含量[J].中国药事,2012,26(1):25-27. |
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| 作者姓名： | 常星洁 刘志辉 严冬 傅静娟 |
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| 作者单位： | 1. 南京中医药大学,南京,210046
2. 南京中医药大学一附院 |
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| 基金项目： | 江苏省中医药局科技项目周期性中药巴布剂外敷治疗乳腺增生病的临床研究(编号LZ09044) |
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| 摘 要： | 目的 建立乳安凝胶膏剂中淫羊藿苷、延胡索乙素等4种化学成分的HPLC测定方法.方法 Hedera C18柱；以乙腈(A)-0.1％磷酸水溶液(B)为流动相进行梯度洗脱,流速:1mL·min-1,柱温:30℃,检测波长:280nm.结果 4种成分均能达到基线分离,各成分在其线性范围内呈良好的线性关系,加样回收率在95％～105％.结论 上述方法可用于乳安凝胶膏剂的含量测定.
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| 关 键 词： | 淫羊藿苷 延胡索乙素 丁香酚 桂皮醛 含量测定 |
Simultaneous Determination of Four Chemical Components in Ru-an Cataplasm by HPLC |
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| Chang Xingjie
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Liu Zhihui
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Yan Dong
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Fu Jingjuan.Simultaneous Determination of Four Chemical Components in Ru-an Cataplasm by HPLC[J].Chinese Pharmaceutical Affairs,2012,26(1):25-27. |
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| Authors: | Chang Xingjie Liu Zhihui Yan Dong Fu Jingjuan |
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| Affiliation: | 1(Nanjing University of Chinese Medicine,Nanjing 210046;1Affiliated Hospital of Nanjing University of Chinese Medicine) |
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| Abstract: | Objective To develop a HPLC method for simultaneous determination of Icariin,corydalis B,eugenol and cinnam aldehyde in Ru-an Cataplasm.Methods The chromatographic separation was achieved on a Hedera C18 column with a mobile phase which was composed of acetonitrile(A) and 0.1% phosphoric acid(B) for gradient elution.The flow rate was 1.0mL·min-1.The column temperature was 30℃,and the detection wavelength was set at 280nm.Results The results showed that four effective components were separated well and showed good linearity.The average recoveries were between 95%-105%.Conclusion The method can be used for the quality control of Ru-an Cataplasm. |
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| Keywords: | Icariin corydalis B eugenol cinnam aldehyde determination |
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