Effect of toosendanin on invasion and migration of human ovarian cancer cells |
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Authors: | LI Yu-ying ZHANG Ke-na CAI Jin-wei LIN Jiang-tao SHAO Xi-ying |
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Affiliation: | 1. Department of Medicine, Quzhou College of Technology Quzhou 324000, China;2. Zhejiang Cancer Hospital, Hangzhou 310022, China;3. Zhejiang Chinese Medical University, Hangzhou 310053, China;4. Quzhou Kecheng Hospital, Quzhou 324000, China;5. Zhejiang University School of Medicine, Hangzhou 310058, China |
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Abstract: | AIM: To investigate the effect of toosendanin (TSN) on invasion and migration abilities of human ovarian cancer cells and the related mechanism. METHODS: The human ovarian cancer cell lines CAVO-3 and SKVO-3 were treated with TSN at different concentrations. The cell viabilty at 12, 24, 48, 72 and 96 h after TSN treatment was measured by CCK-8 assay. Scratch wound healing assay and Transwell assay were employed to measure the invasion and migration abilities of CAVO-3 cells. The protein expression of nuclear factor-κB (NF-κB) p65, E-cadherin, N-cadherin, vimentin and Snail was determined by Western blot. RESULTS: TSN significantly inhibited the viability of CAVO-3 and SKVO-3 cells (P<0.05). Compared with control group, the migration and invasion abilities of CAVO-3 cells in TSN group decreased significantly (P<0.05). In addition, the expression of NF-κB p65 and E-cadherin protein increased notably, followed with N-cadherin, vimentin and Snail protein decreased significantly (P<0.05). However, the inhibitor of NF-κB BAY11-7082 reversed the impact above. Compared with TSN group, the migration and invasion abilities in TSN+BAY11-7082 group increased significantly (P<0.05). The protein expression of E-cadherin also decreased notably, followed with the protein expression of N-cadherin, vimentin and Snail increased significantly (P<0.05). CONCLUSION: TSN inhibits the invasion and migration abilities of human ovarian cancer cells, which is related to the inhibition of epithelial-mesenchymal transition process mediated by NF-κB/Snail signaling pathway. |
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Keywords: | Toosendanin Ovarian cancer Cell invasion Cell migration Epithelial-mesenchymal transition Nuclear factor-κB |
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