阿尔泰金莲花浸膏粉对PM2.5致大鼠急性肺损伤的保护作用

目的：研究阿尔泰金莲花浸膏粉（TAEP）对PM2.5致大鼠急性肺损伤（ALI）的保护作用。方法：60只SD大鼠随机分为正常对照组（生理盐水），模型组，TAEP 125、250、500 mg/kg组，地塞米松（2 mg/kg）阳性对照组，每组10只。各剂量组按相应剂量每天灌胃1次给予受试物，连续30 d，除正常对照组外，其余各组根据预实验结果，在第30天按14 mg/kg行气管滴注PM2.5（采样于乌鲁木齐市）悬液，造成大鼠急性肺损伤。造模3 h后，经大鼠腹主动脉取血，进行白细胞分类计数并检测血清中乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、丙二醛（MDA）、一氧化氮（NO）、白介素-6（IL-6）、白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）的含量；取大鼠右肺上叶作病理组织学检查，下叶称取质量后计算肺湿/干质量比（W/D），取大鼠左肺行肺泡灌洗，并收集肺泡灌洗液（BALF），检测其中IL-6、IL-1β、TNF-α的含量。结果：与正常对照组比较，模型组大鼠外周血白细胞计数，血清和BALF中IL-6、IL-1β、TNF-α含量升高（P均 < 0.01），血清中LDH、MDA、NO含量升高（P均 < 0.01），肺组织W/D升高（P均 < 0.01），SOD和GSH含量降低（P均 < 0.01）；与模型组比较，TAEP各剂量组大鼠外周血白细胞计数，血清和BALF中IL-6、IL-1β、TNF-α含量降低（P均 < 0.05或0.01），血清中LDH、MDA、NO含量降低（P均 < 0.05或0.01），肺组织W/D降低（P均 < 0.05或0.01），SOD和GSH含量升高（P均 < 0.05或0.01）；TAEP 500 mg/kg组各项检测指标与地塞米松组接近（P均 > 0.05），病理检查结果显示随TAEP剂量增加大鼠肺泡形态结构破坏降低，水肿及炎症细胞浸润减少，TAEP 500 mg/kg组大鼠肺脏可见少量炎细胞浸润，肺泡及各级支气管均结构完整，与地塞米松组相近。结论：TAEP对PM2.5致大鼠急性肺损伤具有一定的保护作用。

Effects of Trollius altaicus extract powder on PM2.5-induced lung injury in rats

OBJECTIVE:To study protective effect of Trollius altaicus extract powder (TAEP) on PM2.5-induced acute lung injury (ALI) in rats. METHODS:Sixty SD rats were randomly divided into normal control (normal saline),model (normal saline),TAEP 125 mg/kg,250 mg/kg,500 mg/kg,and dexamethasone positive control (2 mg/kg) groups. Each dose group was orally administered with the test substance for 30 days. Except the normal control group,the other groups were instilled with PM2.5 suspension on the 30th day,causing acute lung injury in rats. Blood samples were collected from the abdominal aorta. Leukocyte count was performed and serum lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione(GSH) and malondialdehyde(MDA) were analyzed. Nitric oxide(NO),interleukin-6(IL-6),interleukin-1β(IL-1β),TNF-alpha(TNF-α) were measured. The right upper lobe was taken for histopathological examination,the lower lobe was weighed to calculate the wet/dry weight ratio (W/D),the left lung was taken for alveolar lavage,and the alveolar lavage fluid (BALF) was collected to detect the contents of IL-6,IL-1beta and TNF-alpha. RESULTS:Compared with the normal control group,the white blood cell count,IL-6,IL-1β and TNF-α levels in serum and BALF were increased (P < 0.01),and serum LDH,MDA and NO levels were increased (P < 0.01),the W/D of lung tissue increased (P < 0.01),and the content of SOD and GSH decreased (P < 0.01). Compared with the model group,the white blood cell count of each dose of TAEP,IL-6 and IL in serum and BALF -1β,TNF-α decreased (P < 0.05 or P < 0.01),serum LDH,MDA,NO content decreased (P < 0.05 or P < 0.01),and lung tissue W/D decreased (P average < 0.05 or P < 0.01),SOD and GSH levels increased (P < 0.05 or P < 0.01); TAEP 500 mg/kg group decreased white blood cell count,serum,BALF IL-1β,IL-6,TNF -α content decreased,serum LDH,MDA,NO content decreased,SOD,GSH content increased,lung W/D decreased compared with dexamethasone group (P < 0.05). CONCLUSION:The results indicate that the Trollius altaicus extract powder has a protective effect on PM2.5-induced lung injury in rats.