喘宁软胶囊对多形核粒细胞生成和释放白三烯的抑制作用

目的：探讨喘宁软胶囊（简称喘宁）平喘作用机制。方法：分别采用生物检定法、HPLC法、免疫ELISA法离体测试大鼠多形核粒细胞（PMNs)生成和释放慢反应物质（SRS－A）、白三烯B4（LTB4）、白三烯C4（LTC4）。结果：在离体试验中，喘宁0．1，0．4，1．6，6．4g/L浓度及喘宁4g/kg ig 20d所得血清能明显抑制大鼠PMNs生成和释放SRS－A；喘宁0．025，0．05，0．1g/L及0．1，0．4，1．6g/L浓度呈量效关系分别抑制大鼠PMNs生成和释放LTB4，LTC4。结论：初步证明喘宁有平喘作用，其机制与抑制花生四烯酸（AA）生成和释放白三烯有关。

Inhibitory effect of Chuanning Soft Capsule on production and release of leukotriene generated by polymorphonuclear leukocytes

Object To explore the anti-asthmatic mechanism of Chuanning Soft Capsule (CNSC). Methods The slow reaction substance of anaphylaxis (SRS-A), leukotriene (LTB 4) and LTC 4 products in rat polymorphonuclear leukocytes (PMNs) were determined by bioassay, HPLC and ELISA in vitro. Results CNSC 0.1, 0.4, 1.6, 6.4 g/L and the serum extracted from rats ig with 4 g/kg CNSC for 20 days significantly reduced SRS-A products in rat PMNs. CNSC 0.025, 0.05, 0.1 g/L and 0.1, 0.4, 1.6 g/L markedly inhibited LTB 4, LTC 4 products in rats PMNs. Conclusion The anti-asthmatic mechanism of CNSC may be correlated with suppression of leukotriene products generated by arachidonic acid.