枯草芽孢杆菌Pab02喷雾剂对肉鸡呼吸道菌群的影响

选取48羽7日龄艾维茵肉公鸡随机分为对照组和试验组,试验组动物房内每日8时喷雾1mL/m3的枯草芽孢杆菌Pab02制剂（108 CFU/mL）,对照组同法喷雾100mL蒸馏水。28日龄时对肉鸡测体质量,采用活菌计数法和PCR-DGGE法对28,49日龄肉鸡呼吸道菌群进行检测,石蜡组织切片法对肺部结构进行观察。结果显示,喷雾枯草芽孢杆菌Pab02对28日龄肉鸡的采食量、日增重、料肉比等生长性能有所提高,但不显著（P〉0.05）;喷雾Pab02能增加49日龄呼吸道细菌数量（P〈0.01）,提高同一生态位菌群的稳定性和呼吸道菌群的多样性;Pab02喷雾剂不引起肺组织损伤,能使淋巴小结增多。结果表明,芽孢杆菌Pab02喷雾剂不能显著改善肉鸡的生长性能,但是能调节呼吸道菌群的结构,增强肺脏的免疫功能。

Application of spraying agent of Bacillus subtilis Pab02 in broilers

Forty eight 7-day-old male avian broilers were randomly divided into the control group and the treatment group.The room of the treatment group was sprayed with 1 mL/m3 of the spraying agent of B.subtilis Pab02(108CFU/mL) every day at 8:00.At the age of 28-day-old,the broilers were weighted.Respiratory tract flora was detected by the method of total viable count and PCR-DGGE at the age of 28 days and 49 days.The structure of the lung tissue of chick was observed by using the routine method of wax section.The results showed that the spraying agent of B.subtilis Pab02 improved the feed intake and the daily gain,reduced the feed gain ratio at 28 d(P>0.05).It increased the number of bacteria at 49 d(P<0.01),improved stability of the microflora at the same niche and the microflora diversity in respiratory tract.Lung tissue was not damaged by the spraying agent of B.subtilis Pab02;it improved the number of the lymphoid nodule.It could be concluded that the spraying agent of B.subtilis Pab02 improved slightly the performance of broilers,regulated respiratory tract flora,and stimulated the immune function of lung.