| ERK1/2信号通路在OX-LDL诱导的血管平滑肌细胞TLR4 mRNA表达中的作用 |
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| 引用本文： | 徐斌,张延斌,许旭光,王大杰,潘德峰,李东野.ERK1/2信号通路在OX-LDL诱导的血管平滑肌细胞TLR4 mRNA表达中的作用[J].徐州医学院学报,2010,30(2):86-88. |
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| 作者姓名： | 徐斌 张延斌 许旭光 王大杰 潘德峰 李东野 |
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| 作者单位： | 徐州医学院附属医院心内科,江苏,徐州,221002 |
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| 摘 要： | 目的探讨ERK1/2信号通路在氧化性低密度脂蛋白（OX-LDL）诱导的血管平滑肌细胞（VSMCs）Toll样受体-4（TLR4）mRNA表达中的作用。方法采用贴块法培养大鼠VSMCs,在氧化低密度脂蛋白（OX-LDL）及PD98059（ERK1/2特异性抑制剂）作用下采用RT-PCR检测VSMCs TLR4 mRNA的表达,用Westernblotting检测ERK1/2磷酸化水平的变化。结果OX-LDL使VSMCs ERK1/2磷酸化水平升高;PD98059抑制ERK1/2的磷酸化;OX-LDL刺激VSMCs上调TLR4 mRNA的表达（P〈0.05）;PD98059预孵育后TLR4 mRNA的表达较单独OX-LDL刺激情况下降低（P〈0.05）。结论OX-LDL通过或部分通过ERK1/2信号通路介导VSMCs TLR4 mRNA的表达。
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| 关 键 词： | 动脉粥样硬化 血管平滑肌细胞 氧化性低密度脂蛋白 Toll样受体-4 ERK1/2 |
Role of ERK1/2 pathway in the oxidized low density lipoprotein induced TLR4 mRNA expression in vascular smooth muscle cells |
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| XU Bin,ZHANG Yanbin,XU Xuguang,WANG Dajie,PAN Defen,LI Dongye.Role of ERK1/2 pathway in the oxidized low density lipoprotein induced TLR4 mRNA expression in vascular smooth muscle cells[J].Acta Academiae Medicinae Xuzhou,2010,30(2):86-88. |
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| Authors: | XU Bin ZHANG Yanbin XU Xuguang WANG Dajie PAN Defen LI Dongye |
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| Affiliation: | XU Bin,ZHANG Yanbin,XU Xuguang,WANG Dajie,PAN Defen,LI Dongye (Department of Cardiology,Affiliated Hospital of Xuzhou Medical College,Xuzhou,Jiangsu 221002,China) |
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| Abstract: | Objective To investigate the role of ERK1/2 pathway in the Toll-like receptor 4(TLR4) mRNA expression in vascular smooth muscle cells(VSMCs) induced by oxidized low density lipoprotein(OX-LDL).Methods VSMCs were isolated from the thoracic aorta of rats by explant technique.TLR4 mRNA expression in VSMCs stimulated with OX-LDL and PD98059(ERK1/2 specific inhibitor) were detected by RT-PCR.The variations of phosphorylation levels of ERK1/2 were detected by Western blotting.Results OX-LDL up-regulated the phosp... |
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| Keywords: | ERK1/2 |
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