| 体外培养海马神经干细胞的分化及超微结构观察 |
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| 引用本文： | 吕楠,蒋莉,刘官信. 体外培养海马神经干细胞的分化及超微结构观察[J]. 医学争鸣, 2009, 30(16): 1448-1451 |
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| 作者姓名： | 吕楠 蒋莉 刘官信 |
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| 作者单位： | 重庆医科大学儿童医院神经内科,重庆,400014;重庆医科大学儿童医院研究所,重庆,400014 |
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| 基金项目： | 国家自然科学基金(30672217) |
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| 摘 要： | 目的：研究胎鼠海马神经干细胞（NPCs）体外培养方法、了解其分化及超微结构特点．方法：分离胎鼠海马NPCs，应用机械分离法将海马组织制成单细胞悬液，用含有bFGF和EGF的无血清培养基培养，传代后用含血清培养基促分化，免疫细胞化学方法对NPCs及其分化后的子代神经细胞进行鉴定，并在电镜下观察分化后细胞的形态结构特征．结果：海马NPCs在无血清培养基中悬浮成球，贴壁后，细胞逐渐从细胞球向周围迁移分化．体外培养胎鼠海马NPCs表达巢蛋白（nestin），在体外可诱导分化产生分别表达Tuj-1和GFAP的神经细胞．电镜观察到分化后的神经细胞及突起相互间排列、具有各种细胞器结构，可见细胞间紧密连接，完整的突触结构．结论：采用无血清培养基中加入特定生长因子的培养技术，可培养出在体外稳定增殖并有多向分化潜能的大鼠胚胎NPCs．
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| 关 键 词： | 神经干细胞 海马 细胞培养技术 超微结构 |
Observation of differentiation and ultrastructure of neural progenitor cells from embryonic rat hippocampus in vitro |
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| Affiliation: | Lü Nan1,JIANG Li1,LIU Guan-Xin2 1Department of Neurology,Children's Hospital,2Institute of Children's Hospital,Chongqing Medical University,Chongqing 400014,China |
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| Abstract: | AIM:To explore the methods of isolation and culture of neural progenitor cells(NPCs)from hippocampus of embryonic rats and to observe the characteristics of induced differentiation and ultrastructure of NPCs.METHODS:Primary NPCs were isolated from Wistar rat(E15-16 d)hippocampus tissues and the cells were suspended and cultured in serum free medium(containing bFGF and EGF)for long-term survival in vitro,and were induced to differentiate in medium containing fetal calf serum.Immunocytochemical staining was a... |
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| Keywords: | neural stem cells hippocampus cell culture techniques ultrastructure |
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