Symplastic continuity in Agrobacterium tumefaciens-induced tumours |
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Authors: | Pradel K; Ullrich C; Cruz S; Oparka K |
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Affiliation: | Unit of Cell Biology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK; Institut fur Botanik, Technische Universitat Darmstadt, Schnittspahnstrasse 3, D-64287 Darmstadt, Germany; Corresponding author |
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Abstract: | The Agrobacterium tumefaciens-induced plant tumour is
regarded as a strong sink, containing a well-developed vascular system that
guarantees an efficient supply of water and nutrients from the host plant
into the tumour. The phloem transport and unloading of the fluorescent dye
carboxyfluorescein (CF) was studied to examine the potential pathways for
unloading of a low-molecular-mass solute, and was compared with the
symplastic movement of potato virus X expressing a green fluorescent
protein-coat protein fusion (PVX.GFP-CP). The distribution of both CF and
PVX.GFP-CP in the host plant, Nicotiana benthamiana,
demonstrated a clear symplastic pathway between the phloem of the host stem
and the cells of the tumour, and also a considerable capacity for
subsequent cell-to-cell transport between tumour cells. This same pattern
of CF transport was also demonstrated independently for the host species
Cucurbita maxima and Ricinus
communis. In addition to entering the tumour, CF and PVX both
moved through the vascular rays of the host stem towards the stele. The
results confirm that host and tumour tissues in the
Agrobacterium gall are in direct symplastic continuity
and emphasize an important symplastic pathway for radial solute transport
in stems.Key words: Agrobacterium tumefaciens,
carboxyfluorescein, GFP, symplastic phloem unloading, plant tumour,
vascular rays
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