AN3CA和BMDC1细胞染色体着丝粒点变异的研究

目的探讨染色体着丝粒点（Cd）的变异与AN3CA细胞和BMDC1细胞染色体非整倍性畸变的关系。方法肿瘤细胞株传代后用常规方法制备染色体,用直接法制备胚胎绒毛组织染色体标本,采用Cd-NOR同步银染分析技术研究AN3CA细胞和BMDC1细胞染色体Cd的变异。结果 1.AN3CA细胞染色体Cd缺失率为1.87%、Cd迟滞复制率为0.19%、小Cd率为1.52%、Cd-NOR融合率为1.88%、与正常人胚胎绒毛细胞染色体Cd相比较,AN3CA细胞染色体Cd缺失、Cd-NOR融合显著升高（P〈0.0125）,而小Cd、Cd迟滞复制其差异无统计学意义。2.BMDC1细胞染色体Cd缺失率为1.13%、Cd迟滞复制率为1.52%、小Cd率为1.53%、Cd-NOR融合率为0.57%、与正常人胚胎绒毛细胞染色体Cd相比较,BMDC1细胞染色体Cd缺失、Cd迟滞复制显著升高（P〈0.0125）,而小Cd、Cd-NOR融合其差异无统计学意义。结论 1.AN3CA细胞染色体非整倍性畸变可能主要源于Cd缺失、Cd-NOR融合;2.BMDC1细胞染色体非整倍性畸变可能主要源于Cd缺失、Cd迟滞复制。

Study on centromeric dots variation of AN3CA cells and BMDC1 cells

Objective： To study the relation between the centromeric dots （Cd） variation of AN3CA cells and BMDC1 cells and the chromosome aneuploidy aberration. Methods： Chromosome of tumor cells were done with general method, Chromosome of normal embryonic villi were done straightly. A Cd-NOR in-phase argentum dye analysis technique modified by this laboratory was used to study centromeric dots （Cd） variation of AN3CA cells and BMDC 1 cells. Results： 1. Frequency of Cd loss in AN3CA cells was 1.87%, Frequency of Cd duplication laggard was 0.19%, Frequency of little Cd was 1.52%, Frequency of Cd-NOR amalgamation was 1.88%. Frequency of Cd loss and Cd-NOR amalgamation in AN3CA cells were significantly higher than those of normal embryonic villi （P〈0.0125） . Frequency of Cd duplication laggard and little Cd showed no difference between AN3CA cells and normal embryonic villi cells. 2. Frequency of Cd loss in BMDC1 cells was 1.13%, Frequency of Cd duplication laggard was 1.52%, Frequency of little Cd was 1.53%, Frequency of Cd-NOR amalgamation was 0.57%. Frequency of Cd loss, Cd duplication laggard in BMDC 1 cells were significantly higher than those of normal embryonic villi cells （P〈0.0125） . Frequency of little Cd, Cd-NOR amalgamation showed no difference between BMDC1 cells and normal embryonic villi. Conclusion： 1.Cd loss and Cd-NOR amalgamation might be related with aneuploidy aberration of AN3CA cells. 2.Cd loss and Cd duplication laggard might be related with aneuploidy aberration of BMDC 1 cells.