MTRR基因A66G多态性与子痫前期发生的相关研究

目的研究蛋氨酸合成酶还原酶(MTRR)基因A66G多态性与子痫前期发生的关系。方法采用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)法对32例子痫前期,70例正常妊娠女性MTRR的A66G进行基因分析。比较上述各组基因型和等位基因频率分布有无差异。结果MTRR基因A66G突变型等位基因G频率在实验组和对照组中有显著性差异,GG基因型频率分布差异有显著性(P<0.05)。AG基因型比AA基因型发生子痫前期风险高1.98倍,GG基因型比AA基因型发生子痫前期风险高5.2倍。结论MTRR A66G基因多态性与子痫前期发生相关,AG、GG基因型增加了子痫前期的发生风险。

Study on the relationship between genetic polymorphisms in the methionine synthase reductase(MTRR) A66G and Preeclampsia

Objective: To explore the relationship between genetic polymorphisms in methionine synthase reductase(MTRR),a central enzyme in folate metabolism,and the risk of Preeclampsia.Methods: Genomic DNA was isolated from the peripheral lymphocytes of 32 patients with Preeclampsia and 70 age matched control subjects.Polymerase chain reaction and restriction fragment length polymorphism were used to examine the polymorphisms of MTRR genes A66G and the relation between these genotypes and risk of Preeclampsia was analyzed.Results: It was obvious difference between experiment and control group in MTRR gene A66G mutation allele(G) frequency(P<0.05),AA and GG had obvious difference(P<0.05).The individuals with 66AG genotype or 66GG genotype had a 1.98 fold or 5.2 fold increased risk of developing Preeclampsia compared with those who had 66AA genotype.Conclusion: MTRR gene mutation allele is related to Preeclampsia,GG,AG gene mutation type increased the risk of Preeclampsia.