人类ermap基因在脐血单个核细胞向红细胞系分化发育过程中表达的研究

为探讨人类红细胞膜相关蛋白（erythroid membrane associated protein，ERMAP）基因在红细胞分化发育过程中的作用，本研究以SCF、IL-3和EPO体外诱导脐血单个核细胞（mononuclear cells，MNCs）向红系细胞方向分化，在此过程中用光学显微镜观察细胞形态，用联苯胺染色计数细胞阳性率，流式细胞术检测CD36^＋／CD235a^-、CD36^＋／CD235a^＋、CD36^-／CD235a^＋细胞的比例，同时以荧光定量PCR（fluorescent quantitative PCR，FQ—PCR）检测人类ermap基因表达量的变化。结果表明，SCF、IL-3和EPO可诱导脐血MNCs向红系方向分化，在此过程中人类ermap基因的表达量不断增加。结论：人类ermap基因与红细胞分化发育密切相关

Expression of Human ermap Gene in Umbilic Cord Blood Mononuclear Cells during Differentiation and Develpment towards Erythroid Lineage

The aim of study was to explore the potential of human erythroid membrane associated protein (ERMAP) gene in erythroid cell differentiation and development, mononuclear cells (MNCs) were isolated from umbilical cord blood and induced to erythroid cell differentiation by SCF, IL-3 and EPO. The cell morphology was observed by using optical microscopy, the positive rate of cells was counted by biphenylamine staining and the ratios of CD36+/CD235a-, CD36+/CD235a+, CD36-/CD235a+ cells were detected by flow cytometry, the change of human ermap gene expression level was analyzed by using fluorescent quantitative PCR (FQ-PCR). The results showed that the ermap gene expression level increased while MNCs were induced to erythroid lineage after treatment with SCF, IL-3 and EPO. It is concluded that the human ermap gene plays an important role in differentiation and development of erythroid cells.