| 外源添加亮氨酸提高乳酸乳球菌酸胁迫抗性 |
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| 引用本文： | 张梦汝,张娟,堵国成,陈坚.外源添加亮氨酸提高乳酸乳球菌酸胁迫抗性[J].食品与生物技术学报,2015,34(2):134-139. |
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| 作者姓名： | 张梦汝 张娟 堵国成 陈坚 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室;江南大学生物工程学院;江南大学糖化学与生物技术教育部重点实验室;江南大学粮食发酵工艺与技术国家工程实验室 |
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| 基金项目： | 国家自然科学基金项目(31470160);国家863计划项目(2011AA100901);国家973计划项目(2013CB733902);中国博士后科学基金项目(2013M540538) |
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| 摘 要： | 在乳酸乳球菌生长过程中外源添加浓度为10mmol/L的亮氨酸,可有效提高乳酸乳球菌在酸胁迫环境下的酸耐受性。在酸性环境中(pH5.0),添加亮氨酸的菌株的生物量为对照菌株的1.24倍;经过酸胁迫(pH4.0)5h后,添加亮氨酸菌株的存活率是对照菌株的28.5倍。进一步的研究表明,亮氨酸的添加可提高胞内NH4+浓度,有效的维持酸胁迫环境下胞内pH(pHin)的稳定,并有效维持乳酸脱氢酶(LDH)的活性,从而有效提高了乳酸乳球菌对酸胁迫的抵御能力。
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| 关 键 词： | 乳酸菌 亮氨酸 酸胁迫 酸胁迫抗性 |
Exogenous Leucine Improved the Acid Tolerance of Lactococcus lactis Under Acid Stress |
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| ZHANG Mengru,ZHANG Juan,DU Guocheng and CHEN Jian.Exogenous Leucine Improved the Acid Tolerance of Lactococcus lactis Under Acid Stress[J].Journal of Food Science and Biotechnology,2015,34(2):134-139. |
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| Authors: | ZHANG Mengru ZHANG Juan DU Guocheng and CHEN Jian |
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| Affiliation: | ZHANG Mengru;ZHANG Juan;DU Guocheng;CHEN Jian;Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University;School of Biotechnology,Jiangnan University;Key Laboratory of Carbohydrate Chemistry & Biotechnology,Ministry of Education,Jiangnan University;National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University; |
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| Abstract: | The addition of leucine was proved to improve the tolerance of Lactococcus lactis NZ9000 to acid stress. When cultivated with leucine under acid stress(pH 5.0),the biomass of L. lactis NZ9000(Leu+) increased 1.24 fold,compared to L. lactis NZ9000(Leu-) (without leucine addition). After challenged at pH 4.0 for 5 h,the survival rate of L. lactis NZ9000(Leu+) increased 28.5 fold,compared to L. lactis NZ9000(Leu-). Further measurements indicated that the addition of leucine could improve the concentration of intracellular NH4+,help to maintain the intracellular pH(pHin) at a relatively high level and protect the activity of lactate dehydrogenase(LDH). Therefore,the addition of leucine could enhance the acid tolerance of L. lactis. |
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| Keywords: | lactic acid bacteria leucine acid stress acid tolerance |
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