神经干细胞移植治疗大鼠创伤性脑外伤的实验研究

目的 将神经干细胞(NSCs)移植于大鼠创伤性脑损伤部位,观察NSCs对创伤性脑损伤的治疗作用.方法 首先进行NSCs的体外培养,同时采用5-2-溴脱氧尿嘧啶(BrdU)标记,脑外伤模型采用自由落体撞击大鼠左侧大脑皮质运动感觉区来制作,脑外伤后24h内移植NSCs,分别于脑外伤前、脑外伤后1、2、3周时行神经运动行为学评分(NMFE)和免疫组织化学染色检测BrdU、巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)和半乳糖脑苷酯(GalC)蛋白表达和TUNEL原位杂交染色,经计算机图像分析系统处理.结果 大鼠自由落体撞击伤后,右下肢神经运动功能障碍明显.神经运动行为学评分在第1周时,实验组与对照组无明显差异,而在第2、3周时,实验组的评分明显低于对照组,有统计学意义(P＜0.05).第2、3周时实验组NSE、GFAP和GalC染色阳性细胞数要多于对照组,nestin染色在第1周时表达最高,而后逐渐下降.BrdU阳性细胞在损伤灶中心区最多,在损伤灶的远隔部位也有少许发现,而TUNEL染色则对照组明显比NSCs组高,有统计学意义(P＜0.05).结论 脑外伤移植NSCs可以在损伤区存活、增殖及分化,并在损伤的远隔部位有少许NSCs迁移、分化.NSCs移植有利于大鼠脑自由落体撞击伤后期的功能恢复.

Effects of embryonic neural stem cells on rat brain weight--dropping injury

Objective To investigate the effects of embryonic neural stem cells(NSCs)on the repair of rat brain weight-dropping injury.Methods NSCs were cultured in vitro,labeled with BrdU,and transplanted into rat brain area injured by weight-dropping.The neurological motor function evaluation(NMFE)were used to test the functions at the 1,2,3 weeks post-injury.Immunohistochemistry was used to detect the expression of BrdU,nestin,NSE,GFAP and TUNEL.Results There was significant difference in NMFE between experiment group and control group(P<0.01)at 2,3 weeks.At 2,3 week,there were more expression of NSE,GFAP and GalC in experiment group than that in control group.Expression of nestin and TUNEL was higher at 1 week than that at 2,3 week.BrdU positive cells were mainly detectable in traumatic area and simultaneously less at the distance from traumatic area using immunohistochemical staining.Conclusion NSCs have effects on rat brain weight-dropping injury.Transplanted NSCs can survive,proliferate,differentiate and immigrate in traumatic area.