响应面法优化鸡枞菌多糖的提取工艺

利用响应面分析法优化鸡枞菌多糖提取工艺.以鸡枞菌多糖提取率为指标,考察浸提温度、时间、液固比、提取次数对鸡枞菌多糖提取的影响,然后根据中心组合( Box-Benhnken)原理采用三水平三因素的分析法,依据回归确定各工艺条件的影响因素,以鸡枞菌多糖提取率为响应面和等高线,分析各个因素的显著性和交互作用,优化得到鸡枞菌多糖的最佳工艺条件为提取温度87℃、提取时间2.9h、水料比31∶1(mL/g),多糖最大提取率达16.88％.

Optimization of Extraction Conditions for Polysaccharides from Collybia albuminosa via Response Surface Methodology

The extraction technology of polysaccharides from Collybia albuminosa was optimized via response surface methodology(RSM).Using extraction rate of polysaccharide from Collybia albuminosa as the indicator,effects of extraction temperature,time,water-material ratio and extraction times on the extraction rate of polysaccharide were investigated.Based on single factor studies,a three-variable,three-level Box-Benhnken experimental design and response surface methodology were used to obtain the optimized extraction conditions of polysaccharides.The optimum conditions of extraction technology of polysaccharide from Collybia albuminosa were extraction temperature of 87 ℃,extraction time of 2.9 h and water-material ratio of 31:1(mL/g),under which the extraction rate of polysaccharide was up to 16.88%.