伤风净喷鼻液体外抗病毒作用的实验研究

目的　观察伤风净喷鼻液在体外的抗病毒作用。方法　先测定药物对细胞的毒性 ,观察细胞病变Cy topathceffect(CPE) ,找出最大无毒浓度 ;再测定流感病毒FM1株、合胞病毒Long株的病毒滴度。最后测定伤风净喷鼻液抑制各病毒对细胞产生细胞病变 (CPE)的作用。结果　实验表明伤风净喷鼻液抗流感病毒FM1株的最低有效浓度为1∶10 2 4,相当于伤风净喷鼻液原药浓度 1.0 5 5mg/ml ,抗合胞病毒Long株的最低有效浓度为 1∶40 96,相当于伤风净喷鼻液原药浓度 0 .2 64mg/ml。 结论　根据实验结果证明伤风净喷鼻液具有明显的抗流感病毒FM1株、合胞病毒Long株作用

Experimental study of the antivirus effect of Shangfengjing nasal - spray liquid in vitro

Objective To observe the antivirus effects of Shangfengjing nasal-spray liquid in vitro. Methods First the toxicity on cell was determined, the cytopathiceffect(CPE) was observed tofind the maximal innocuous dose. Then the titers of the influenza virus FM 1 strain and syncytial virus Long strain was determined. At last the inhibitory effect on CPE induced by the virus was determined. Results The study showed that the minimal effective dose on influenza virus FM 1 strain was 1∶1 024, which was equal to 1.055 mg/ml original liquid;the minimal effective dose on syncytial virus Long strain was 1∶4 096, which was equal to 0.264 mg/ml original liquid. Conclusions The results showed that the Shangfengjing nasal-spray liquid had obvious antivirus effect on influenza virus FM 1 strain and syncytial virus Long strain.