胃癌组织中E-钙黏蛋白、MMP-2蛋白和MMP-9蛋白的表达及意义

目的探索胃癌组织及癌旁组织中E-钙黏蛋白、基质金属蛋白酶-2（MMP-2）及基质金属蛋白酶-9（MMP-9）的表达情况，并分析其表达与胃癌临床病理特征的关系。方法采用ABC免疫组化染色方法检测40例胃癌组织和癌旁组织中E-钙黏蛋白、MMP-2蛋白及MMP-9蛋白的表达情况，比较2种组织中3种蛋白的表达情况；并分析该3种蛋白与胃癌临床病理特征的关系。结果胃癌组织与癌旁组织比较，其E-钙黏蛋白的表达下调（P〈0．05），而MMP．2蛋白和MMP．9蛋白表达均上调（P〈0．05）。T3＋T4期、N1～N3期及Ⅲ＋Ⅳ期胃癌组织中E-钙黏蛋白的表达阳性率分别低于T1＋T2期、NO期及Ⅰ＋Ⅱ期胃癌组织（P〈0．05）；MI期和Ⅲ＋Ⅳ期胃癌组织中MMP-2蛋白的表达阳性率分别高于M0期和I＋Ⅱ期胃癌组织（P〈0．05）；T3＋T4期、Ⅲ＋Ⅳ期及低-未分化的胃癌组织其MMP．9蛋白的表达阳性率分别高于T1＋T2期、I＋Ⅱ期及高-中分化胃癌组织（P〈O．05）。结论E-钙黏蛋白可能参与胃癌的浸润及淋巴结转移，MMP-2蛋白可能参与胃癌的远处转移，而MMP-9蛋白可能参与胃癌的分化及肿瘤浸润。该3种蛋白可作为判断胃癌分期和预后的指标。

Expression and Significance of E-cadherin,MMP-2 Protein,and MMP-9 Protein in Gastric Cancer Tissues

[Abstract] Objective To explore the expressions of E-eadherin, matrix metalloproteinases-2 （MMP-2） protein, and matrix metalloproteinases-9 （MMP-9） protein in gastric cancer tissues, and to analyze the possible statistical relati- onship between the expressions ofE-cadherin, MMP-2 protein, and MMP-9 protein, and clinicopathological features of gastric cancer. Methods The ABC immunohistochemical staining was adopted to examine the expressions of E-cadherin, MMP-2 protein, and MMP-9 protein in 40 paraffin slices of gastric cancer （gastric cancer group）, with the adjacent tissue as the control group （adjacent tissue group）. The positive rates of 3 kinds of protein were compared between the 2 groups, in addition, the statistical relationship between the expressions of the 3 kinds of protein and clinicopathological features of gastric cancer was examined respectively by SPSS 19.0 software. Results The expressions of E-cadherin, MMP-2 protein, and MMP-9 protein were all found in gastric cancer tissues and adjacent tissues. In gastric cancer tissue group, the expression of E-cadherin downregulated while the expressions of MMP-2 protein and MMP-9 protein upregulated in comparison to adjacent tissue group （P〈0. 05）. The significant association was found between the expre- ssion of E-cadherin and the gastric cancer tissues of T3 ＋ T4 stage, N1-N3 stage, and Ⅲ＋Ⅳ stage, which had lower positive expression rate （P〈 0. 05）. The expression of MMP-2 protein in gastric cancer tissues of M1 stage and Ⅲ＋Ⅳ stage upregulated （P〈 0. 05）, and the expression of MMP-9 protein upregulated in gastric cancer tissues ofT3 ＋ T4 stage, Ⅲ＋Ⅳ stage, or lowlydifferentiated＋undifferentiated （P〈0.05）. No significant relationship was found in other clinicalpathological features and 3 kinds of protein except aforementioned significant relationship （P〉 O. 05）. Conclusions In the development progress of gastric cancer, the E-cadherin may get involved in the mechanism of tumor invasion and lymph node metastasis, MMP-2 protein may get involved in the mechanism of distant metastasis, and MMP-9 protein may get involved in the mechanism of differentiation and tumor invasion. The examination of those 3 kinds of markers may play an role in the judgment of tumor stage and estimation of prognosis in gastric cancer clinically.