| 高效液相色谱法测定柴藿颗粒中黄芩苷的含量 |
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| 引用本文： | 李姝梅,阳敬,熊磊,韦静,霍剑明,高文全,李甲英,李安华. 高效液相色谱法测定柴藿颗粒中黄芩苷的含量[J]. 儿科药学杂志, 2012, 18(3): 43-45 |
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| 作者姓名： | 李姝梅 阳敬 熊磊 韦静 霍剑明 高文全 李甲英 李安华 |
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| 作者单位： | 云南中医学院,云南昆明,650500 |
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| 基金项目： | 云南省教育厅科学研究基金重大专项项目 |
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| 摘 要： | 目的:建立柴藿颗粒中黄芩苷的含量测定方法.方法:采用高效液相色谱法,选用Diamonsil C18(2)色谱柱(250 mm×4.6mm,5μm),流动相为甲醇-0.2％磷酸(46∶54),检测波长280 nm,流速1.0 mL/min,进样量5μL,柱温30℃.结果:黄芩苷在0.632 ～3.792μg范围内线性关系良好,回归方程为Y=3E+06X-481319(r=1,n=6),平均加样回收率101.46％,RSD为2.14％.结论:本法简便快捷、灵敏,结果准确,可用于柴藿颗粒中黄芩苷的含量测定.
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| 关 键 词： | 高效液相色谱法 黄芩苷 柴藿颗粒 |
An HPLC Method for the Determination of Baicalin in Chaihuo Particles |
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| Li Shumei , Yang Jing , Xiong Lei , Wei Jing , Huo Jianming , Gao Wenquan , Li Jiaying , Li Anhua. An HPLC Method for the Determination of Baicalin in Chaihuo Particles[J]. Journal of Pediatric Pharmacy, 2012, 18(3): 43-45 |
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| Authors: | Li Shumei Yang Jing Xiong Lei Wei Jing Huo Jianming Gao Wenquan Li Jiaying Li Anhua |
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| Affiliation: | (Yunnan University of Traditional Chinese Medicine,Yunnan Kunming 650500,China) |
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| Abstract: | Objective: To establish a determination method of baicalin in Chaihuo Particles.Methods: A mobile phase of methanol-0.2% phosphoric acid(46∶54) was pumped at 1 mL/min through a Diamonsil C18(2) chromatographic column(250 mm×4.6 mm,5 μm).Detection wavelength was 280 nm.Sample volume was 5 μL,and the column temperature was 30 ℃.Results: There was a good linear relationship within the range of 0.632~3.792 μg.The regression equation was Y=3E+06X-481319(r=1,n=6).The average recovery rate was 101.46%,and RSD was 2.14%.Conclusions: This method is simple,fast,sensitive and accurate,which can be used in the determination of baicalin in Chaihuo Particles. |
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| Keywords: | High performance liquid chromatography Baicalin Chaihuo particles |
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