| 多维液相色谱-质谱用于酶解猪血蛋白中活性肽的研究 |
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| 引用本文： | 张维冰,孟繁琼,张庆合,李彤.多维液相色谱-质谱用于酶解猪血蛋白中活性肽的研究[J].分析试验室,2006,25(10):36-39. |
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| 作者姓名： | 张维冰 孟繁琼 张庆合 李彤 |
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| 作者单位： | 齐齐哈尔大学,齐齐哈尔,161006;中国科学院大连化学物理研究所,大连,116023;齐齐哈尔大学,齐齐哈尔,161006;大连依利特分析仪器有限公司,大连,116011;大连依利特分析仪器有限公司,大连,116011;中国科学院大连化学物理研究所,大连,116023 |
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| 摘 要： | 以强阳离子交换柱（SCX）为一维色谱柱，反相柱（RP）为二维色谱柱，采用在线捕集接口形式，通过10通阀连接一、二维色谱柱，构建了二维液相色谱分离系统。将该系统用于酶解猪血蛋白中对血管紧缩素Ⅰ转移酶（ACE）具有活性抑制作用的肽进行分离、鉴定，共检测出104个组分。收集一维馏分，离线注入LC—MS，鉴定出其中含有SAL、DKF、ESF、STVL及FESF5个小肽。
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| 关 键 词： | 多维液相色谱 质谱 活性肽 |
| 文章编号： | 1000-0720（2006）10-036-04 |
| 收稿时间： | 2005-12-30 |
| 修稿时间： | 2005-12-302006-02-21 |
Application of multidimensional liquid chromatography tandem mass spectrometry in the analysis of bioactive peptides derived from pig blood hydrolyzed by pepsin |
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| ZHANG Wei-bing,MENG Fan-qiong,ZHANG Qing-he,LI Tong.Application of multidimensional liquid chromatography tandem mass spectrometry in the analysis of bioactive peptides derived from pig blood hydrolyzed by pepsin[J].Chinese Journal of Analysis Laboratory,2006,25(10):36-39. |
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| Authors: | ZHANG Wei-bing MENG Fan-qiong ZHANG Qing-he LI Tong |
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| Affiliation: | 1. Qiqihar University, Qiqihar 161006; 2. Dalian Elite Analytical Instruments Co., Ltd., Dalian 116011; 3. Dalian Institute of Chemical Physics, CAS, Dalian 116023 |
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| Abstract: | A multidimensional liquid chromatography coupled to mass spectrometry separation system was developed for the identification of bioactive peptides derived from pig blood hydrolyzed by pepsin.A strong cation-exchange chromatography(SCX) worked as the first phase followed by reverse phase chromatography(RP) worked as the second phase,a trap column was incorporated between the two phases of chromatography and coupled by a two-position,ten-port valve;The system resolved more than 104 peaks.The fraction from the SCX was gathered and analyzed by liquid chromatography coupled to electrospray ionization(ESI) mass spectrometry.According to their UV and mass spectra,5 of them were preliminarily identified as SAL,DKF,ESF,STVL,FESF. |
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| Keywords: | Multidimensional liquid chromatography Mass spectrometry Bioactive peptides |
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