Mcl-1反义寡核苷酸对肝癌细胞增殖及凋亡的影响

目的:探讨髓样细胞白血病-1(Mcl-1)反义寡核苷酸(ASODN)对肝癌HepG2细胞增殖及凋亡的影响。方法:将Mcl-1 ASODN转染入体外培养的肝癌HepG2细胞。用WST-8法检测不同浓度的Mcl-1 ASODN对HepG2细胞增殖抑制率;用流式细胞仪检测Mcl-1 ASODN对HepG2细胞周期和凋亡的影响,用Hoechst33258染色在荧光显微镜下观察HepG2细胞凋亡的形态学改变。结果:Mcl-1 ASODN作用HepG2细胞48 h后,与空白对照组(blankcontrol)和随机寡核苷酸(RODN)对照组相比,Mcl-1 ASODN组能明显抑制肝癌HepG2细胞的增殖和诱导其凋亡(P<0.05),Mcl-1 ASODN组细胞出现S期明显的阻滞;Hoechst33258染色可见大量的细胞核固缩,核碎裂。结论:Mcl-1 ASODN能抑制肝癌细胞的增殖,诱导细胞凋亡。

Effect of antisense oligodeoxynucleotide targeting Mcl-1 on proliferation and apoptosis of hepatocarcinoma cells

Aim:To investigate the effect of antisense oligodeoxynucleotide targeting Mcl-1 on proliferation and apoptosis of HepG2 cells. Methods: Antisense oligodeoxynucleotide targeting Mcl-1 was transfected into HepG2 cells incubated in vitro.The inhibitory rate of proliferation was quantified by WST-8 test;The effect of antisense oligodeoxynucleotide targeting Mcl-1 on cell cycle and apoptosis of HepG2 cells was tested by flow cytometry,the morphology of apoptosis were observed by Hoechst 33258 staining under fluorescence microscope.Results: After treated with antisense oligodeoxynucleotide targeting Mcl-1 48 hours,compared with blank control and random oligodeoxynucleotide groups,antisense oligodeoxynucleotide targeting Mcl-1 can significantly suppressed the growth of hepatocarcinoma cells and induce their apoptosis(P<0.05),antisense oligodeoxynucleotide targeting Mcl-1 induce significant increase in s-phase cells,many HepG2 cells stained with Hoechst 33258 exhibited apoptotic morphology,such as cell shrinkage,nuclear condensation and nuclear fragmentation.Conclusion:Antisense oligodeoxynucleotide targeting Mcl-1 can inhibit the proliferation and induce the apoptosis of hepatocarcinoma cells in vitro.