Slug对照射鼠肠上皮细胞的保护作用及机制

 目的：研究Slug过表达对照射鼠肠上皮细胞的保护作用及机制 方法：以体外培养的小肠隐窝上皮细胞IEC6和转染了Slug cDNA的IEC6为研究对象，对细胞进行6Gy剂量γ射线照射，在照射48h后观察细胞凋亡指数，免疫组化和Western blot法检测PUMA蛋白表达。结果：6Gy射线照射IEC6细胞48h后的细胞凋亡指数为15.6+1.54，明显高于照射联合pcDNA3-Slug cDNA转染组的5.1+1.7（P<0.01）和单独pcDNA3-Slug cDNA转染组的2.21+0.36（P<0.01）。Weatern blot法检测发现，6Gy射线照射IEC6细胞48h后的PUMA相对表达为0.79+0.12，明显高于照射联合pcDNA3-Slug cDNA转染组的0.16+0.01（P<0.01），免疫组化和Weatern blot法检测结果一致。结论：上调Slug抑制射线诱导的细胞PUMA表达上调，抑制IEC6细胞凋亡。

The protection effect and mechanism of Slug on irradiation of rat intestinal epithelial cells

Objective To investigate the protection effect and mechanism of slug overexpression on irradiation of rat intestinal epithelial cells.Methods The intestinal crypt epithelial cells(IEC6) were used and transfected with Slug cDNA in vitro.The cells were irradiated with 6 Gy 60Co gamma rays.48 hours after the irradiation,apoptosis indexes were calculated.The expression of PUMA was observed by the immunohistochemistry and Western blot. Results The apoptosis indexes in IEC6 by the irradiation of 60Co gamma rays after 48 hours were significantly higher than that in pcDNA3-Slug cDNA and in pcDNA3-Slug cDNA without irradiation(15.60±1.54 versus 5.10±1.70;2.21±0.36,P<0.01).The expression of PUMA in IEC6 by the irradiation of 60Co gamma rays after 48 hours was significantly higher than that in pcDNA3-Slug cDNA by Western-blot(0.79±0.12 versus 0.16±0.01,P<0.01).The immunohistochemistry and Western blot assay results were consistent.Conclusions Upregulation of Slug can restrain the upregulation expression of PUMA in irradiated cells and the apoptosis of IEC6.